African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Prokaryotic expression of vitellogenin receptor gene of Actias selene Hubner

Yingying Xu, Baojian Zhu, Qiuning Liu, Guoqing Wei, Li Chen,S Lei Yu and Chaoliang Liu*
College of Life Science, Anhui Agricultural University, 130 Changjiang West Road 230036, Peoples Republic of China.
Email: [email protected]

  •  Accepted: 04 May 2012
  •  Published: 05 June 2012

Abstract

Vitellogenin receptor plays a key role in the embryonic development of oviparous animals. A vitellogenin receptor (VgR) gene was cloned from Actias selene using reverse transcriptase-polymerase chain reaction and rapid amplification of cDNA end PCR. Sequence analysis revealed that this gene was 5848 bp and encoded a protein of 1812 amino acids peptide with high similarity to Bombyx mori VgR. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and western blotting analysis demonstrated that a 24 and 40 KD recombinant proteins corresponding to the ligand-binding domains of A. selene VgR was successfully expressed in Escherichia coli cells. 

 

Key words: Actias selene Hubner, vitellogenin receptor (VgR), prokaryotic expression.