African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Endosulfan induced alterations in physiological responses in Lycopersicum esculentum seeds during germination

CHIBI Fatiha* and SAYAH Fouad
Pôle d’excellence Régional-Centre des Etudes Environnementales Méditerranéennes (CEEM), Laboratoire de Biologie Appliquée and Sciences de l'Environnement, Université Abdelmalek Essaâdi. Faculté des Sciences et Techniques, BP 416, Tanger, Maroc.
Email: [email protected], [email protected]

  •  Accepted: 26 June 2011
  •  Published: 19 December 2011

Abstract

 

The impact of endosulfan (an organochlorine insecticide), at four concentrations (0.015, 0.03, 0.045 g/L and its recommended field rate 0.06 g/L) on some morphological and biochemical parameters was investigated during the germination process of tomato seeds. The seeds germinating in presence of endosulfan had less and later emergence than the control seeds; the insecticide caused a decrease in both roots and shoots length. Protein patterns showed an over expression in all seeds exposed to endosulfan treatment during germination. Even at low concentration (0.015 g/L), six fold higher esterase activity was recorded compared to control. The same effect was shown on protease activity which was three fold greater compared to control at concentration of 0.06 g/L; however, a significant inhibition of α-amylase activity was detected. At concentration of 0.015 g/L, the activity was around 60% compared to control.  On the other hand, an increase in proline levels was observed in response to endosulfan application.

 

Key words: Insecticide, endosulfan, tomato, seed germination.