The present study was carried out with the aim of bio-securing of white spot syndrome virus (WSSV) onPenaeus monodon culture in grow-out and laboratory condition during the period of March to August 2010. In grow-out system, thirty six earthen ponds of varying sizes ranging from 0.5 to 1.0 ha were surveyed. Among 36 ponds, based on the differences in the management techniques, 15 were semi-intensive (group-I), 10 ponds were modified extensive (group-II) and 11 ponds as extensive (group- III). The results of the present study revealed that maximum average body weight (34.5 g), survival of 88% and proportion of pond success was 100% (15/15) with average culture period of 127 days in semi-intensive with probiotics applied ponds and the minimum average body weight (25.2 g), survival of 79.1% and the proportion of pond failure was 27.2 (3/11) with average culture period of 70.7 days in extensive with regular management. In laboratory experiment, it showed that the cumulative mortalities occurred on 77th day in treatment-I and in control on 45th day and proportion of tanks failure was 100% (14/14) with average culture period of 70.7 days. Histopathological observation also revealed that hypertrophied nuclei was very low in 50 day of experiment than that of 77th day of experiment.
Key words: Histopathology, polymerase chain reaction, Penaeus monodon, probiotics, white spot syndrome virus.
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