African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Evaluation of candidate reference genes for real time quantitative PCR normalization in pear fruit

  Tao Wu, Ruiping Zhang, Chao Gu, Juyou Wu, Hongjian Wan, Shujun Zhang and Shaoling Zhang*
  College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China, 210095.
Email: [email protected]

  •  Accepted: 06 June 2012
  •  Published: 03 July 2012

Abstract

 

In this study, eight candidate reference genes including EF1αTUB-b2GAPDH18S rRNARP-L27PUBI,ACT and ACT2 were selected to evaluate expression stability in pear fruit of different genotypes, tissues, developmental stages and phytohormone-treatments, using three software approaches: geNorm, NormFinder and BestKeeper. The results showed that EF1αTUB-b2 and GAPDH had the most stable expression across all experimental conditions tested. EF1α and TUB-b2 were the most stable reference genes across all genotypes. GAPDH and EF1α were the most suitable reference genes across different tissues. For developmental stage and phytohormone-treated samples, TUB-b2 and GAPDH were the most stable reference genes.

 

Key words: qPCR, reference gene, pear.