African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Anti-atherogenic properties associated with the antioxidant activity from the hydrophilic extracts of Halimeda incrassata (Chlorophyta, Bryopsidales)

Alexis Vidal-Novoa
  • Alexis Vidal-Novoa
  • Department of Biochemistry, Faculty of Biology, University of Havana, Cuba.
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Ariadna Costa-Mugica
  • Ariadna Costa-Mugica
  • Department of Biochemistry, Faculty of Biology, University of Havana, Cuba.
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Yenisleidy Zulueta-Díaz
  • Yenisleidy Zulueta-Díaz
  • Department of Biochemistry, Faculty of Biology, University of Havana, Cuba.
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Daylín Diaz-Gutierrez
  • Daylín Diaz-Gutierrez
  • Department of Biochemistry, Faculty of Biology, University of Havana, Cuba.
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Ana Mara de Oliveira e Silva*
  • Ana Mara de Oliveira e Silva*
  • Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Brazil.
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Ana María Vazquez
  • Ana María Vazquez
  • Faculty of Pharmacy, Université de Montréal, Canada.
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Claudina Zaldívar-Munoz
  • Claudina Zaldívar-Munoz
  • Department of Biochemistry, Faculty of Biology, University of Havana, Cuba.
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Dalva Assuncao Portari de Mancini
  • Dalva Assuncao Portari de Mancini
  • Institute Butantan, Sao Paulo, Brazil.
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Jorge Mancini-Filho
  • Jorge Mancini-Filho
  • Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Brazil.
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  •  Received: 12 November 2014
  •  Accepted: 29 October 2016
  •  Published: 26 January 2017

Abstract

Seaweeds are a source of natural antioxidants having potential application in oxidative stress and associated diseases. In this work, anti-atherogenic properties associated with the antioxidant activity from the hydrophilic extracts of Halimeda incrassata were studied. The phenolic content assessed in the aqueous extract and fraction phenolic acids (FPA) was 0.13 ± 0.05 and 0.47 ± 0.09 mg of gallic acid equivalents (GAE)/g dry seaweed, respectively. In DPPH•, radical scavenging assay fractions exhibited a dependent concentration. The seaweeds extract inhibited the desoxirribose oxidation in the presence or absence of EDTA (IC50 = 1.91± 0.09 mg/mL) (IC50 = 2.95 ± 0.01 mg/mL). In vivo antioxidant properties of FPA-H.incrassata were investigated in rats with a CCl4-induced liver injury. Pre-treatment with H. incrassata led to approximately 50% reductions in liver TBARS levels.  The treatment with H. incrassata FPA also increased the activity of the CAT enzyme, which in turn resulted in an enhanced antioxidant defense. The expression of Catalase by PCR-RT technique demonstrated a higher gene expression when compared with that which was observed in the CCl4-treated group. Antiatherogenic properties were studied in the inhibition of lipoprotein oxidation mediated by Cu2+ or HRP/H2O2, free radical scavenging, and metal ion chelation, and it was dose dependent with a higher concentration needed for the aqueous extract than for the FPA fraction. Antioxidant activity was also improved in macrophages as evaluated in the cell supernatant (by TBARS formation); and by luminol enhanced chemiluminescence after cell activation with zymosan; and a degree of cell lipoperoxidation was decreased by the Halimeda incrassata extract. The results of this work add to the antioxidant potential of the seaweed for its application in oxidative stress associated conditions.

Key words: Antiatherogenic properties, antioxidant activity, seaweeds, Halimeda incrassate.