African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Effects of genotypes and medium on callus induction of proso millet derived from anther culture

  Limin Wu1#, Tianyu Yang2,3#, Guojun Dong4, Jihong He3, Kongjun Dong3, Huizhong Wang1 and Yanchun Yu1*        
  1College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China. 2College of Life and Sciences Technology, Gansu Agricultural University, Lanzhou 730070, China. 3Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China. 4State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang, 310006, China.
Email: [email protected]

  •  Accepted: 22 September 2012
  •  Published: 30 November 2012

Abstract

 

Proso millet is a promising species for rich nutrition and drought tolerance. Factors influencing anther-derived callus induction efficiency were investigated when microspores of anthers taken at the late-uninucleate to early- binucleate stage were used as explants. The results indicate that callus induction is strongly affected by genotype. Several highly responsive cultivars of proso millet with desired traits have been identified that could be directly used in crop improvement by breeding. Cold pre-treatment of spikes for three weeks, followed by 32.5°C heat treatment for a short time significantly improved callus yield. Moreover, W14 basal medium and a combination of 60 g/L sucrose with 60 g/L maltose as carbon source were optimal for anther culture of proso millet in this study. To our knowledge, this is the first report on successful regeneration of plants from pollen-derived callus in proso millet for use as a source to accelerate breeding programs.

 

Key words: Proso millet, temperature, medium, genotype, carbohydrate.