African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Superovulation and embryo quality with porcine follicle stimulation hormone (pFSH) in Katahdin hair sheep during breeding season

Fernando Sánchez1*, Hugo Bernal1,  Alejandro S. del Bosque1, Adán González1, Emilio Olivares1, Gerardo Padilla3, Rogelio A. Ledezma2
1Universidad Autónoma de Nuevo León, Department of Agronomy, Laboratory of Animal Reproduction, Agricultural Sciences Campus UANL, Francisco Villa S/N, Ex Hacienda Canadá, P.O. Box 66050 General Escobedo, Nuevo León, México. 2Department of Veterinary Medicine, Laboratory of Reproduction, Agricultural Sciences Campus UANL, Francisco Villa S/N, Ex Hacienda Canadá, P.O. Box 66050 General Escobedo, Nuevo León, México. 3School  of Medicine,Agricultural Sciences Campus UANL, Francisco Villa S/N, Ex Hacienda Canadá, P.O. Box 66050 General Escobedo, Nuevo León, México.  
Email: [email protected]

  •  Accepted: 11 June 2013
  •  Published: 20 June 2013

Abstract

Estrus synchronization of 21 ewes was carried out using intravaginal sponges (60 mg of medroxyprogesterone acetate, Sincrogest®-Sanfer, Mexico) for 14 days. 7 ewes were randomly assigned to one of the three treatments: T1, 80 mg; T2, 120 mg; T3, 140 mg of porcine follicle stimulating (pFSH) (Folltropin-p®-Bioniche, Canada) administered on day 12 after sponge insertion. The dose was divided in decreasing doses; second and third day representing 50 to 75% and 25 to 33% of the first day dose, respectively. At the beginning of superovulation treatment, each ewe received 5 mg of dinoprost tromethamine (1 ml of Lutalyse® Pfizer, Mexico). An effect of pFSH level was observed on the interval from sponge removal and the onset of estrus (REI) (P < 0.01), with a difference of 13.5 h between ewes from treatment 2 and 3. The number of follicles (F), number of collected embryos (CE) and transferable embryos (TE) (P < 0.05) was also different among treatments, with highest values observed in T3 (3.0 ± 0.6 for CE and 2.3 ± 0.5 for TE). Embryo recovery rate was in average 66.5%, (P > 0.05) of CL observed. Further investigation is guaranteed to evaluate the effect of pFSH doses on estrus synchronization for selected breeding seasons.

 

Key words: Embryo transfer, hair sheep, porcine follicle-stimulating hormone (pFSH), breeding season.