The susceptibility of the bean beetle Callosobruchus maculatus to Metarhizium anisopliae var. anisopliae(URM3349) and Metarhizium anisopliae var. acridum (URM4412) strains was assessed at concentrations of 108, 107, 106, 105 and 104 conidia.ml-1 under laboratory conditions. The beetles were raised in glass recipients on a natural diet of cowpeas (Vigna unguiculata). Concentrations caused mortality in adult insects. At the concentration of 108 conidia ml-1, mortality of C. maculatus caused by M. anisopliae var.anisopliae and M. anisopliae var. acridum was 74.45 and 58.27%, respectively. The LC50 of M. anisopliaevar. anisopliae was estimated at 9.2 × 103 conidia ml-1. The results demonstrated that the fungal strains evaluated have pathogenic action against the bean beetles under laboratory conditions.
Key words: Entomopathogenic fungus, Metarhizium anisopliae, pathogenicity, Callosobruchus maculatus, mortality
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