African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Susceptibility of Callosobruchus maculatus (Coleoptera: Bruchidae) to strains of Metarhizium anisopliae

  Carlos Fernando Rodrigues Guaraná1, Francisco Braga da Paz Júnior2*, Eliana Santos Lyra da Paz3 and Elza Aurea Luna-Alves Lima4
  1Department of Biology, Federal Rural University of Pernambuco, CEP: 52171-900, Recife, PE, Brazil. 2Federal Institute of Education, Science and Technology of Pernambuco, CEP: 50740-540, Campus Recife, PE, Brazil. 3Faculty of Dentistry of Pernambuco, University of Pernambuco, CEP: 54.753-220 Camaragibe, Pernambuco, Brazil. 4Department of Mycology, Federal University of Pernambuco, CEP: 50670-420, Recife, PE, Brazil.
Email: [email protected]

  •  Accepted: 16 February 2012
  •  Published: 17 July 2012

Abstract

 

The susceptibility of the bean beetle Callosobruchus maculatus to Metarhizium anisopliae var. anisopliae(URM3349) and Metarhizium anisopliae var. acridum (URM4412) strains was assessed at concentrations of 108, 107, 106, 105 and 104 conidia.ml-1 under laboratory conditions. The beetles were raised in glass recipients on a natural diet of cowpeas (Vigna unguiculata). Concentrations caused mortality in adult insects. At the concentration of 108 conidia ml-1, mortality of C. maculatus caused by M. anisopliae var.anisopliae and M. anisopliae var. acridum was 74.45 and 58.27%, respectively. The LC50 of M. anisopliaevar. anisopliae was estimated at 9.2 × 103 conidia ml-1. The results demonstrated that the fungal strains evaluated have pathogenic action against the bean beetles under laboratory conditions.

 

Key words: Entomopathogenic fungus, Metarhizium anisopliae, pathogenicity, Callosobruchus maculatus, mortality