African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Cloning and gene expression analysis of ascorbic acid biosynthesis enzymes in Moringa oleifera

Takayuki Kondo
  • Takayuki Kondo
  • Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
  • Google Scholar
Yukichi Fujikawa
  • Yukichi Fujikawa
  • Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
  • Google Scholar
Akihiro Ueda
  • Akihiro Ueda
  • Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
  • Google Scholar
Toshinori Nagaoka
  • Toshinori Nagaoka
  • Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
  • Google Scholar
Hirofumi Saneoka
  • Hirofumi Saneoka
  • Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
  • Google Scholar
Milton Martinez Gonzalez
  • Milton Martinez Gonzalez
  • Faculty of Science, Autonomous University of Santo Domingo, Santo Domingo, 10105, Dominican Republic.
  • Google Scholar
Manuel Calcano
  • Manuel Calcano
  • Faculty of Science, Autonomous University of Santo Domingo, Santo Domingo, 10105, Dominican Republic.
  • Google Scholar
David Hernandez-Martich
  • David Hernandez-Martich
  • Faculty of Science, Autonomous University of Santo Domingo, Santo Domingo, 10105, Dominican Republic.
  • Google Scholar
Muneharu Esaka
  • Muneharu Esaka
  • Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan.
  • Google Scholar


  •  Received: 04 November 2014
  •  Accepted: 19 May 2015
  •  Published: 28 May 2015

Abstract

Moringa (Moringa oleifera), which is a semi-tropical plant, is used as food and for the production of medicines and oil products, because of a large amount of various nutrients including ascorbic acid (AsA). Although Moringa leaf has a high AsA content, the molecular mechanisms of AsA accumulation in Moringa have received little attention. In this study, we isolated Moringa cDNAs for enzymes, belonging to the major AsA biosynthesis pathway (Smirnoff-Wheeler pathway) in higher plants. The predicted amino acid sequences showed 70% or more similarity to those of Arabidopsis. Quantitative RT-PCR indicated that Moringa GDP-L-galactose phosphorylase (GGP) is most highly expressed in Moringa during leaf development and light exposure. A significant high promoter activity of the Moringa GGP gene was detected by promoter assay in Arabidopsis protoplast.

 

Key words: Moringa oleifera, ascorbic acid, biosynthesis enzymes, gene expression.