This study was carried out to determine which system of milkings and sampling methodology presenting chemical composition results safer and lower variation of the results. In Properties I and II, the fresh milk samples were collected in 40 ml flasks containing bronopol in order to compare the chemical composition of the milk, as adopting two sample collection methods, that is two and three milkings. In order to compare the chemical composition of milk and SCC, between two sampling methodologies, in two daily milkings (Property I) and three daily milkings (Property II), fresh milk samples were collected randomly totaling 300 samples on the property I and 312 samples on the property II. The milk chemical components were determined through analytical principle that is based on differential absorption of infrared waves by components of the milk. By analyzing the treatments, experimental design was used entirely randomized and averages were compared by Tukey test using the statistical program SISVAR (statistical analysis system). When evaluating the data obtained through variation coefficient (VC), the occurrence of experimental was observed, since most results showed low variation. However the distinct procedures for sampling of the fresh milk may present varied levels of the chemical composition and SCC, therefore overestimating or underestimating the values of the milk composition, when samples are collected at different times of the day and different number of milkings.
Key words: Cows of high production, isolates samples, period, pool, sampling methodology.
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