African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Effect of indolebutyric acid (IBA) and naphthaleneacetic acid (NAA) plant growth regulaters on Mari gold (Tagetes erecta L.)

Zia Ullah1, Sayed Jaffar Abbas1,2*, Nisar Naeem1, Ghosia Lutfullah2, Taimur Malik1, Malik Atiq Ullah Khan1 and Imran Khan1
1Biotechnology Center, Agricultural Research Institute, Tarnab, Peshawar, Pakistan. 2Center of Biotechnology and Microbiology, University of Peshawar, Pakistan.
Email: [email protected]

  •  Accepted: 15 May 2013
  •  Published: 01 August 2013

Abstract

This experiment was conducted for the optimization of auxin [indolebutyric acid (IBA) and naphthaleneacetic acid (NAA)] required for the regeneration of Mari gold. Significant differences were observed for bloom flowers. The maximum branches per plant (6.8) were observed at 400 ppm. Maximum flower size (10.7) was exhibited at 100 ppm IBA concentration and minimum flower size (2.8) at 300 ppm. The 200 and 400 ppm IBA concentration showed maximum leaf size (4.0). The maximum leaves per plant (44.0) were observed at 100 ppm IBA concentration while 200 ppm showed minimum leaves per plant (6.0). At 100 ppm IBA concentration maximum plant height (9.40 cm) was observed and minimum plant height (5.92 cm) was recorded at 400. 200 and 300 ppm concentration of IBA have no significant effect on roots per plants (37.0) while 400 ppm has maximum effect on roots per plant (84.4). Root size increased (6.50) at 100 ppm IBA concentration, while increasing the IBA concentration decreases the root size (4.10) respectively. Maximum value (7.2) was observed at 100 ppm IBA for non-bloom flower and minimum value was (3.2) at 300 ppm. Maximum branches per plant (5.0) were recorded at 400 ppm NAA concentration followed by 300 ppm (4.0) while minimum value (3.2) was observed at 200 ppm. The three concentration of NAA 100, 300 and 400 ppm are significantly different from control for flower size and there is no difference between control and 200 ppm concentration of NAA. By dipping the seedling of Mari gold in NAA maximum leaf size (3.20) was recorded at 200 ppm concentration, while minimum leaf size was observed at 400 ppm. Increased in leaves per plant were observed with increase in NAA concentration. Maximum plant height (7.80) was recorded at 100 and 200 ppm NAA concentration while minimum value was (5.0) at 300 ppm concentration. By dipping the seedling in higher concentration of NAA showed the maximum increase in roots per plant (123.2) and root size (6.8). 300 ppm showed maximum value for non-bloom flower and 200 ppm showed the minimum value (3.2).

 

Key words: Gold, indolebutyric acid (IBA), naphthaleneacetic acid (NAA, auxins.