African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali

Adounigna Kassogue
  • Adounigna Kassogue
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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Kadia Maiga
  • Kadia Maiga
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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Diakaridia Traore
  • Diakaridia Traore
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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Amadou Hamadoun Dicko
  • Amadou Hamadoun Dicko
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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Rokiatou Fane
  • Rokiatou Fane
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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Tiby Guissou
  • Tiby Guissou
  • Institut de l?Environnement et de Recherches Agricoles, 03 B.P. 7047 Ouagadougou, Burkina Faso.
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Fatouma Alhadji Faradji
  • Fatouma Alhadji Faradji
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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Fernando Hercos Valicente
  • Fernando Hercos Valicente
  • Embrapa Milho e Sorgo C.P. 151, CEP: 35701 970 Sete Lagoas, Minas Gerais, Brasil.
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Abdoulaye Hamadoun
  • Abdoulaye Hamadoun
  • Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Science and Technology, University of Sciences, Techniques and Technologies of Bamako, Bamako, BP E320 Mali.
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  •  Received: 26 April 2015
  •  Accepted: 03 June 2015
  •  Published: 09 July 2015

Abstract

The objective of this work was to isolate and characterize Bacillus thuringiensis from agricultural and other insect breeding sites in Mali. A hundred soil samples were collected from Bamako district, Segou, Sikasso and Timbuktu regions. B. thuringiensis (Bt) was isolated from the samples using a heat-acetate method and the isolates were identified and classified using morphological and biochemical tests. The frequency of B. thuringiensis in soils was noted. The results showed that, 15 out of the 3111 bacterial isolates were putative Bt. thuringiensis. Most isolates produced parasporal crystals. The average Bt index for all the areas sampled was 5.1%; the highest frequency was recorded for Niono in Segou region (11.7) and the lowest for Bozola in Bamako district (0.5). Contrary the known information on the high content and distribution of B.  in soils, the agricultural soils of Mali contain few Bt strains, confirmed by the low Bt index obtained.

 

Key words: Bacillus thuringiensis, isolation, agricultural soils, Mali.