African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Effect of different carbon sources on the accumulation of carbohydrate, nutrient absorption and the survival rate of Chinese ash (Fraxinus mandshurica) explants in vitro

Zhengzheng Deng1,2*#, Jianmin Chu3*#, Qingli Wang4 and Lihua Wang1    
1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, People’s Republic of China. 2Graduate University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China. 3Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, People’s Republic of China. 4Kunming Branch, Chinese Academy of Sciences, Kunming 650204, People’s Republic of China.    
Email: [email protected]

  •  Accepted: 23 April 2012
  •  Published: 26 May 2012


In this study, we analyzed the effect of different carbon sources on: (i) carbohydrate and nutrient absorption in Chinese Ash (Fraxinus mandshurica) explants in vitro, and (ii) explant survival rate during ex vitroacclimatization. The explants were cultured under one of five conditions: (1) in a solid medium supplemented with 30 g l-1 sucrose (SM); (2) in a modified temporary immersion bioreactor (TIB) supplemented with sucrose at 0 (TIB0) or (3) 30 g l-1 (TIBS) concentration; (4) in TIB flushed with CO2 at 700 to 900 µl l-1 (TIBC) with no sucrose; or in TIB flushed with CO2 at 700 to 900 µl/l while simultaneously adding sucrose 30 g l-1 (TIBCS). The explants cultured under TIBCS exhibited the highest level of root induction and acclimatization survival rate. In addition, these explants exhibited maximized fresh weights (FW), dry weights (DW), and sucrose contents. Explants cultured in SM showed the highest rate of carbohydrate accumulation. At the end of the culture incubation period, absorption of nitrogen, phosphorus, potassium, magnesium, iron, zinc and manganese was also higher in explants cultured in SM than in TIB. This study revealed that the use of TIBCS provided optimum explant survival.


Key wordsFraxinus mandshurica, temporary immersion bioreactor, CO2 enrichment, nutrient absorption.