African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6688

Full Length Research Paper

In vitro study on the role of the tannins of Newbouldia laevis and Zanthoxylum zanthoxyloides on infective larvae of Trichostrongylus colubriformis

P. A. Olounlade
  • P. A. Olounlade
  • Laboratoire d’Ethnopharmacologie et de Santé Animale (LESA), Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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E. V. B. Azando
  • E. V. B. Azando
  • Laboratoire d’Ethnopharmacologie et de Santé Animale (LESA), Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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E. Y. Attakpa
  • E. Y. Attakpa
  • Laboratoire d’Ethnopharmacologie et de Santé Animale (LESA), Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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J. D. Gbenou
  • J. D. Gbenou
  • Laboratoire de Pharmacognosie et des Huiles essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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G. G. Alowanou
  • G. G. Alowanou
  • Laboratoire d’Ethnopharmacologie et de Santé Animale (LESA), Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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E. Tchétan
  • E. Tchétan
  • Laboratoire d’Ethnopharmacologie et de Santé Animale (LESA), Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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C. C. Dansou
  • C. C. Dansou
  • Laboratoire de Pharmacognosie et des Huiles essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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M. S. Hounzangbe-Adote
  • M. S. Hounzangbe-Adote
  • Laboratoire d’Ethnopharmacologie et de Santé Animale (LESA), Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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F. Gbaguidi
  • F. Gbaguidi
  • Laboratoire de Pharmacognosie et des Huiles essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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M. Moudachirou
  • M. Moudachirou
  • Laboratoire de Pharmacognosie et des Huiles essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin.
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H. Hoste
  • H. Hoste
  • INRA, UMR 1225 Interactions Hôte Agents Pathogènes 23, Chemin des Capelles, F-31076 Toulouse, France.
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A. Valentin
  • A. Valentin
  • Pharma-Dev -UMR 152 / Faculté de Pharmacie, Université de Toulouse III, 35 Chemin des Maraîchers, 31062 Toulouse, Cedex 9 France.
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  •  Received: 28 July 2011
  •  Accepted: 10 October 2017
  •  Published: 14 December 2017

Abstract

Trichostrongylus colubriformis is an important cause of parasitic gastroenteritis in small ruminants which causes diarrhea, weakness, loss of production and death. The in vitro efficacy of tannins of Newbouldia laevis and Zanthoxylum zanthoxyloides was determined against this parasitic nematode. Larvae of T. colubriformis were incubated at 23°C in the leaf extracts of N. laevis and Z. zanthoxyloides at concentrations of 150, 300, 600 and 1200 µg ml-1 for three hours, respectively. Phosphate buffered saline (PBS) was used as negative controls. Inhibition of larval migration was significantly (P < 0.05) observed with increasing concentrations of the extracts. In the range of concentration examined, the results were dose-dependent for N. laevis but not for Z. zanthoxyloides. The addition of Polyvinyl polypyrrolidone (PVPP) inhibited total or part of the anthelmintic effect. These results suggest that the larval migration is at least in part due to the action of tannins and supports the traditional use of N. laevis and Z. zanthoxyloides against parasites nematodes. Further research is required to isolate and structurally identify the active anthelmintic compounds, and to improve methods of plant extraction of the effective anthelmintic components that will be readily adaptable for use by rural communities against helminthiasis.

 

Key words: Larval migration, tannins, Trichostrongylus colubriformis, Newbouldia laevis, Zanthoxylum zanthoxyloides, Benin.