African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

A new process to promote the use of controlled mycorrhization practice in forest nurseries

I. Ndoye
  • I. Ndoye
  • Laboratoire Commun de Microbiologie IRD / ISRA / UCAD, Centre de Recherche de Bel Air, BP 1386, Dakar. Sénégal.
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A. Soumaré
  • A. Soumaré
  • Laboratoire Commun de Microbiologie IRD / ISRA / UCAD, Centre de Recherche de Bel Air, BP 1386, Dakar. Sénégal.
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E. C. Agbangba
  • E. C. Agbangba
  • Université Cheikh Anta Diop, Département de Biologie végétale, Laboratoire d’Ecologie végétale et d’Ecohydrologie, Dakar Sénégal.
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J. Thioulouse
  • J. Thioulouse
  • Université de Lyon; CNRS; UMR 5558, Laboratoire de Biométrie et Biologie Evolutive, 43 boulevard du 11 novembre 1918, Villeurbanne F-69622, France.
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A. Galiana
  • A. Galiana
  • CIRAD, UMR 113 CIRAD/INRA/IRD/SUPAGRO/UM2, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM), Montpellier, France.
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Y. Prin
  • Y. Prin
  • IRD. UMR 113 CIRAD/INRA/IRD/SUPAGRO/UM2, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM). Montpellier, France.
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R. Duponnois
  • R. Duponnois
  • IRD. UMR 113 CIRAD/INRA/IRD/SUPAGRO/UM2, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM). Montpellier, France.
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  •  Accepted: 27 November 2012
  •  Published: 31 January 2013

Abstract

 

The aims of this study were to test a new mycorrhizal inoculation process using a “catalyser” of the mycorrhizal establishment (termite mounds of Macrotermes subhyalinus) to minimize the requested volume of fungal inoculum added to the cultural substrate. The effects of the termite mound were explored on mycorrhiza formation between an Australian Acacia, Acacia holosericea and an ectomycorrhizal fungus or an arbuscular mycorrhizal fungus using a two-step cultural system. The first step of this cultural practice was the inoculation of A. holosericea seedlings in small soil volumes (5 L plastic containers planted with 100 pre-germinated seeds) whereas the second one allowed the development of these mycorrhized plants in larger soil volumes (1 L pots planted with one seedling). Termite mound amendment significantly enhanced the mycorrhizal formation from both types of fungal isolates. This stimulating effect could probably be attributed to the introduction via the termite mound of a bacterial group (that is, fluorescent pseudomonads) that could act as Mycorrhiza Helper Bacteria (MHB). Since it is possible to reduce the requested fungal inoculum in controlled mycorrhization practice using M. subhyalinus mound powders, this biotechnological process could be useful in re-afforestation of tropical regions by lowering the requested fungal inoculum quantities and reducing the financial costs of controlled mycorrhization in forest nurseries.

 

Key words: Termitaria, fluorescent pseudomonads, controlled mycorrhization, Acacia holosericea.