African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6574

Full Length Research Paper

Isolation, characterization and screening of Burkholderia caribensis of rice agro-ecosystems of South Assam, India

Biswajit Deb Roy
  • Biswajit Deb Roy
  • Microbiology Laboratory, Department of Life Science, Assam University, Silchar-788011, Assam, India.
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Bibhas Deb
  • Bibhas Deb
  • Microbiology Laboratory, Department of Botany, Gurucharan College, Silchar-788004, Assam, India.
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Gauri Dutta Sharma
  • Gauri Dutta Sharma
  • Microbiology Laboratory, Department of Life Science, Assam University, Silchar-788011, Assam, India.
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  •  Accepted: 08 January 2013
  •  Published: 06 February 2013

Abstract

 

Six isolates of Burkholderia were obtained from rhizosphere soils of rice grown in tropical lowlands of South Assam, India. Among the identified Burkholderia isolates, SDSA-I10/1 has shown higher nitrogen fixing potential and it was selected for 16S rDNA sequencing. The isolate SDSA-I10/1 showed highest resemblance to Burkholderia caribensis MWAP84 (Y17011) and hence, identified as Burkholderia caribensis strain SDSA-I10/1 (GU372342). Inoculation of this strain improved the growth and yield parameters of rice significantly over uninoculated control plants, thus it may be used as indigenous microbial inoculant for intensive rice cropping in tropical lowlands.

 

Key wordsBurkholderia caribensis strain SDSA-I10/1, 16S rDNA sequence, biofertilizer, Diazotroph, nitrogenase activity, tropical lowland.