The anthocyanins in the fruit skin of Pyrus communis cv. ‘Red Du Comice’ were isolated, identified and quantified using high-performance liquid chromatography/diode array detection (HPLC/DAD) and HPLC/electrospray ionization/mass spectrometry (HPLC/ESI/MS). The individual anthocyanins were identified by comparing their mass spectral data and retention times with those of standards and published data. Cyanidin-3-galactoside was the major compound, taking up to 93% of the total anthocyanin content. The content of Cyanidin-3-galactoside reached 23.7 ± 3.2 mg 100 g-1 fresh weight, followed by the eighth peak (2.14%) and Cyanidin-3-glucoside (1.23%). Pelargonidin-3-rutinoside (pg-3-rutinoside) was identified for the first time in P. communis L. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays showed that the extract from the fruit peel of ‘Red Du Comice’ contained anthocyanins and possessed high antioxidant capacity.
Key words: Pyrus communis, anthocyanin, high-performance liquid chromatography-electrospray ionization-mass spectrometry, antioxidant activity.
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