African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Effects of salicylic acid and nitric oxide on antioxidant capacity and proline accumulation in Glycine max L. treated with NaCl salinity

Mehdi Simaei1*, Ramezan Ali Khavari-Nejad1, Sara Saadatmand1, Francoise Bernard2 and Hamid Fahimi1
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.  2Faculty of Biological Sciences, Shahid Beheshti G. C. University, Tehran, Iran.
Email: [email protected]

  •  Accepted: 21 February 2011
  •  Published: 18 August 2011

Abstract

The influence of salicylic acid (SA), sodium nitroprusside (SNP) and their reciprocal effects (SA+SNP) on certain physiological parameters in soybean seedlings grown in saline and non-saline conditions was investigated. The changes of leaf area, shoot fresh, dry weights and content of photosynthetic pigments showed that the addition of 100 µM SA and/or 100 µM SNP markedly declined the oxidative damage to soybean plants treated with NaCl salinity. The results proved that the interaction of salicylic acid with nitric oxide donor significantly enhanced the activities of catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (GPOX). Also, they decreased the amounts of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in soybean leaves under sodium chloride toxicity. In order to reduce the oxidative damage caused by NaCl stress, the protective role of SA+SNP was often better than that of SA and SNP alone. As well, it was observed that the accumulation of proline was apparently accelerated by these substances under salt stress and that the interaction of salicylic acid with nitric oxide has synergistic effects in decreasing the deleterious effects induced by NaCl salinity.

 

Key words: Salicylic acid, nitric oxide, NaCl salinity, soybean.