Root-lesion nematodes are a serious yield-reducing disease of many crops in many parts of the world. The aim of this study was identify populations of Pratylenchus spp. in different soybean fields in the Central region of Brazil based upon sequence analyze of ITS-5.8S rDNA region and comparison on GenBank by BLAST. The results showed that a total of 10 nematodes populations showed similarity with Pratylenchus brachyurus and remainder showed similarity Pratylenchus bolivianus. This is the first report that could indicate the occurrence of mixed populations and a new species of the genus Pratylenchus in soybean crops in Brazil.
Key words: Polymerase chain reaction (PCR), Pratylenchus bolivianus, Pratylenchus brachyurus, ITS region, soybean.
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