African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Identification of Pratylenchus spp. in soybean in Central region of Brazil using the ITS-5.8S rDNA region

Lilianne Martins Ribeiro1*, Hercules D. Campos2, Dauri J. Tessmann1, Cláudia R. Dias-Arieira3, Danilo L. das Neves1 and Cleiltan Novais da Silva1
1Universidade Estadual de Maringá - Programa de Pós-graduação em Agronomia, 87020-900, Maringá, PR, Brazil. 2Universidade de Rio Verde, UniRV, Departamento de Agronomia /Fitopatologia; Caixa Postal 104, 75901-970, Rio Verde, GO, Brazil. 3Universidade Estadual de Maringá – Campus Regional de Umuarama, 87507-190, Umuarama, PR, Brazil.
Email: [email protected]

  •  Accepted: 12 November 2013
  •  Published: 21 November 2013

Abstract

Root-lesion nematodes are a serious yield-reducing disease of many crops in many parts of the world. The aim of this study was identify populations of Pratylenchus spp. in different soybean fields in the Central region of Brazil based upon sequence analyze of ITS-5.8S rDNA region and comparison on GenBank by BLAST. The results showed that a total of 10 nematodes populations showed similarity with Pratylenchus brachyurus and remainder showed similarity Pratylenchus bolivianus. This is the first report that could indicate the occurrence of mixed populations and a new species of the genus Pratylenchus in soybean crops in Brazil.

Key words: Polymerase chain reaction (PCR), Pratylenchus bolivianus, Pratylenchus brachyurus, ITS region, soybean.