African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6578

Full Length Research Paper

Growth and development of Phaius tankervilleae (Banks) Blume when inoculated with orchid mycorrhizal fungi

  Shu-Fen Cheng1,2, Chih-Hsing Yeh3, Chen-Han Jan4 and Doris Chi-Ning Chang1*        
  1Department of Horticulture and Landscape Architecture, National Taiwan University, Taipei, Taiwan 10617, Republic of China. 2Herbarium (TAI), College of Life Science, National Taiwan University, Taipei, Taiwan 10617, Republic of China. 3Taoyuan District Agricultural Research and Extension Station, Council of Agriculture, Executive Yuan, Taoyuan, Taiwan 327, Republic of China. 4Department of Agronomy, National Taiwan University, Taipei, Taiwan 10617, Republic of China.
Email: [email protected]

  •  Accepted: 14 September 2012
  •  Published: 30 November 2012

Abstract

 

Phaius tankervilleae (Banks) Blume is the most attractive and most horticulturally valuable native Phaiusspecies in Taiwan. Due to overharvesting in the wild, however, the plant is on the verge of extinction. Successful cultivation of P. tankervilleae has been achieved through in vitro seeding or tissue culture propagation, but plantlet survival rates are low upon transplantation. Previous studies demonstrated that inoculation with mycorrhizal fungi markedly improved plantlet survival rates, vegetative and reproductive growth in Orchidaceae plants. Four orchid mycorrhizal fungi (OMF), R01 (Rhizoctonia solani), R02 (Rhizoctonia sp., teleomorph = Ceratobasidium sp. AG-A), R15 (Rhizoctonia sp., teleomorph = Ceratobasidium sp. AG-Fb) and R19 (Rhizoctonia sp., teleomorph = Ceratobasidium sp. AG-G) were isolated from Taiwanese native orchid roots that were cultured for more than seven years in our laboratory. These fungi all proved to be non-pathogenic and were inoculated onto the root of P. tankervilleae. Inoculation with the appropriate OMF increased seedling survival rate 27 to 31% (R02; R15), plant height 6.3 cm (R15), leaf length 3.5 cm (R15), flower stalk length 10.2 cm (R19) and number of flowers per stalk (3.5) in ex vitro-grown P. tankervilleae. Inoculation with OMF (R19) promoted reproductive growth in P. tankervilleae, while OMF (R02) inoculation significantly increased photosynthetic rate and carbohydrate content. P. tankervilleae seedlings inoculated with the R15 isolate had a higher survival rate than with other treatments. Inoculations with Rhizoctonia spp. isolates R02, R15, or R19 also promoted reproductive growth in P. tankervilleae.

 

Key words: Pelotons, Phaius tankervilleae, reproductive growth, Rhizoctonia species, survival rate, vegetative growth.