Endophytic bacterial strains were isolated from healthy mountain-cultivated ginseng leaves, and Botrytis cinerea was responsible for the fungus disease. Antagonistic strain was identified based on morphological characteristic and 16S rRNA sequence analysis. The results showed that 56 endophytic bacteria were isolated from healthy mountain-cultivated ginseng leaves, and FS-1 strain has good inhibitory effect on B. cinerea. The antifungal effects of fermentation broth of FS-1 strain on eight plant pathogenic fungi were obvious. The strain FS-1 has very good nitrogen fixation ability and it can produce extracellular protease. The strain FS-1 was identified as Bacillus cereus according to the morphological, physiological and biochemical characteristics, and 16S rRNA sequence analysis. This study suggests that FS-1 strain has certain potential bio-control, and could be useful to effectively control B. cinerea that causes grey mold disease in ginseng.
Key words: Endophytic bacteria, antifungal, 16S rRNA sequence, bio-control.
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