African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Effects of selective medium on lipid accumulation of chlorellas and screening of high lipid mutants through ultraviolet mutagenesis

Xiaodong Deng1, Yajun Li1 and Xiaowen Fei1,2*  
1Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Science, Haikou, 571101, China. 2Department of basic medicine, Hainan medical college, Haikou, 57110, China.
Email: [email protected]

  •  Published: 18 August 2011

Abstract

Two micro-algae Y019 and Y041 were isolated from tropical fresh water in Hainan, China. Throughphylogenetic analysis of 18S rDNA sequences, they had been identified as Chlorella vulgaris and Chlorella pyrenoidosa. Subsequently, both of them and C. vulgaris FACHB-31 were cultured respectively in four selective medium (SE, BG11, HSM1, DS) at 25°C, 230 r/min, 24 h full light[z1], 50-110 μE/m2/s. Effects of these media on the cell growth and lipid content were detected. The results showed that, the HSM1 medium is more suitable for the growth of Chlorella, while the DS medium is more conducive to the lipid accumulation. In the DS medium, the lipid content of C. vulgaris Y019 was 1.84 ~ 2.64-fold than others at 12 days. So the local separated algae C. vulgaris Y019 is thought of an ideal raw material for biodiesel production. After that, we conducted Ultraviolet mutagenesis for C. vulgaris Y019. A total of 1086 algae strains had been screened for lipid production in HSM and HSM-N media.. Results showed: in HSM medium, mutants Y019-M57 and Y019-M66 were identified as high lipid producing mutants, while in HSM-N medium Y019-M67 was detected as high lipid content mutants. These mutants laid the foundation for further research and commercial application.

 

Key words: Chlorella, culture medium, Ultraviolet mutants, lipid accumulation.