African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6576

Full Length Research Paper

Evaluation of lyophilized and non lyophilized toxins from Trichoderma species for the control of Ceratocystis paradoxa

E. I. Eziashi1*, N. U. Uma2, A. A. Adekunle2, C. E. Airede1 and E. E. Odigie1
1Nigerian Institute for Oil Palm Research (NIFOR), Plant Pathology Division, P. M. B. 1030, Benin City, Edo State, Nigeria. 2Department of Botany and Microbiology, University of Lagos, Akoka, Yaba, Lagos State, Nigeria.
Email: [email protected]

  •  Accepted: 13 May 2010
  •  Published: 04 July 2010

Abstract

Lyophilized and non-lyophilized toxins produced from Trichoderma species were investigated against Ceratocystis paradoxa mycelial growth at different concentrations in-vitro. Lyophilized toxin inhibition on C. paradoxa by T. viride(28.14 and 28.14%) and T. polysporum (28.88 and 28.88%) at 70 and 100% concentrations were significant (p = 0.005) compared with T. hamatum (20.37 and 21.48 %) and T. aureoviride (20.00 and 20.37%) six days after incubation. Non-lyophilized phytotoxin inhibition on C. paradoxa mycelia by T. viride (21.85 and 23.335%) and T. polysporum (22.22 and 22.59%) at 70 and 100% were also significant (p = 0.005) when compared with T. hamatum (16.29 and 18.14%) andT. aureoviride (17.03 and 17.77%) 6 days after incubation. The mycelial growth ofC. paradoxa was stimulated by T. hamatum and T. aureoviride at 20 and 50% concentrations with lyophilized and non-lyophilized phytotoxins. The lyophilized phytotoxin exhibited better control of C. paradoxa compared with non-lyophilized and the control treatments. Using gas chromatography-mass spectrometry, column fraction isolated from T. viride Rf value 0.51 was deduced to be 1, 2-benzendicaboxylic acid. It is responsible for the inhibitory action on C. paradocamycelia. This compound produced by T. viride depended on type of fungus strain and standard used.

 

Key words: GC-MS, inhibition, mycelia, 1, 2-benzendicarboxylic acid.