Broadcast and in line application of phosphorus in soils with different densities

The efficiency of broadcast versus in line phosphate fertilization has been studied; however, soil density as a determining factor for soil efficiency has been less studied. Thus, the objective of this study was to evaluate the effect of broadcast and in line phosphate fertilization in soil with different densities on corn development. The experiment was conducted in pots under greenhouse condition. A completely randomized design was used in a 2x3 factorial arrangement with four replications. Treatments were two levels of soil densities (1.2 and 1.6 g cm -3 ) and three forms of P application (broadcast, in line and without P). Corn stem diameter, plant height, root volume, root and shoot dry matter, and root and shoot phosphorus content and accumulation were evaluated. Application in line resulted in higher P accumulation by corn plant. There was higher plant dry matter accumulation at soil density 1.2 g cm -3 than at 1.6 g cm -3 . Phosphate fertilization in line at soil density 1.2 g cm -3 enhances dry matter accumulation in corn plants.


INTRODUCTION
Soil compaction is one of the most severe degradation processes that occurs in the soil and have been affecting about 68 million hectares around the world from the vehicular traffic (Nawaz et al., 2013).Its effect on the soil structure results in higher soil density (Souza et al., 2012) and lower soil porosity (Singh and Hadda, 2014).The availability of nutrients for plants is reduced in compacted soils (Barzegar et al., 2016), such as phosphorus (Novais et al., 2007).Besides that, soil compaction leads to lower crop yield due to increased resistance to root growth and reduced efficiency of water and nutrient use (Twum and Nii-Annang, 2015).
The nutrient P participates on plant metabolism as the main component of adenosine triphosphate (Taiz et al., 2017), this nutrient has an essential role in physiological and biochemical processes, acting as an energy transfer agent within the plant; thus enabling photosynthesis and respiration (Hawkesford et al., 2012), having essential role on plant development (Amanullah and Almas, 2012).
The soils with a high degree of weathering help to maintain low levels of available phosphate resulting from high P adsorption capacity of iron and aluminum oxides and hydroxides in soils (DeLong et al., 2013;Fink et al., 2014).Slow diffusion contributes to low level of available P in soil which limits plant uptake (Shen et al., 2011).Moreover, in line fertilization (close to root system) should be chosen over other methods to favor plant uptake of P (Prado, 2008).
The most common method of P application in grain production is soil surface with or without incorporation, in row placement and in band placement (Galetto et al., 2014).Among the determining factors for the best P application method are soil physical and chemical proprieties, fertilizer type and crop type (Ceretta et al., 2007).The phosphate fertilizer applied on the surface will keep contact with soil, causing the P fixation and affecting the absorption by the plants (Barbosa et al., 2018).
Reports have shown that application of phosphate fertilizers using broadcast method without incorporation in a no till system leads to different distribution of P within the soil profile compared to in line application, this will enhance P concentration in superficial layer from 0 to 5 cm (Souza et al., 2016).However, soil compaction might affect the broadcast phosphate fertilization management in no till areas (Santos et al., 2005), as root development and crop production especially in areas under water deficiency might be reduced (Valadão et al., 2015).
Therefore, it is possible that in line phosphate fertilization has higher efficiency than broadcast application, especially in compacted soils.The objective of this study is to evaluate the effect of broadcast and in line phosphate fertilization in soil with different densities on corn development.

MATERIALS AND METHODS
The experiment was conducted in the greenhouse and in the Laboratory of Soil, Fertilizer and Foliar Analysis (LASAF) of University of Mato Grosso State -UNEMAT (09° 51' 42'' S and 56º 04' 07'' W), campus Alta Floresta -MT, in 2016.According to Köppen classification, the weather is classified as Aw with two well defined seasons: raining summer and dry winter, with temperatures around 26 °C and average rainfall between 2000 and 3000 mm (Alvares et al., 2014).
The experiment was conducted in a completely randomized design in a factorial 2x3 with 4 replications.The treatments were two soil densities D1 1.2 g cm -3 (normal) and D2 1.6 g cm -3 (compacted), without P application (T1), with superficial broadcast P (T2), and with in line P (T3).Each experimental unit was composed using a pot of 4 dm -3 capacity.
Soil density was determined using Beutler and Centurion (2004) methodology.The soil was pressed 15 times with a 3.5 kg wood of 50 cm high and a diameter lower than the 4 dm -3 plot of PVC (17.5 cm of height and 17.3 cm of diameter).The soil was compacted on each 1.7 cm soil layer until the soil densities was 1.2 and 1.6 g cm - 3 .
The soil was dried and sieved before dolomite lime application (with 85 relative efficiency of reactivity -RER) so as to enhance soil base saturation to 60%, as suggested by Sousa and Lobato (2004).The soil was incubated in pots with periodic irrigation for the duration of 90 days.After this period, 200 mg dm -3 of P (Mono-Ammonium-Phosphate -50% of P2O5) was applied to broadcast and in line pots, according to the methodology of Malavolta (1981).In line application was placed under and next to the seed in order to simulate field situation.Corn sowing was done with six seeds per pot.All pots were applied with 60 kg ha -1 of K2O (Potassium chloride -60% of K2O), 20 kg ha-1 of N at sowing and 40 kg ha -1 of N later (urea -45% of N) (Sousa and Lobato, 2004).Boron was applied during sowing at 2 kg ha -1 (boric acid 17% of B) and zinc at 6 kg ha -1 (zinc sulfate -20% of Zn) (Sousa and Lobato, 2004).
Irrigation with tap water was done every day, in order to maintain soil water capacity of about 60%.After 35 days of emergency, the stem diameter, shoot height, root volume, root and shoot dry matter, and root and shoot P content and accumulation were evaluated.
The roots were separated from shoots, washed with tap water followed by distillated water, and left submerged inside a graduated cylinder containing low level of water.The difference between initial and final volume was used to determine root volume.Roots and shoots were placed into Kraft papers and dried in a forcedventilated oven at 65°C for 72 h to determine its dry matter content.Shoots and roots samples were ground in a Wiley grinder and chemically analyzed for P content using Embrapa (2009) methodology.
The results, for normality (Shapiro-Wilk) and homoscedasticity, were analyzed.The analysis of variance (ANOVA) was done with F test (p<0.05),and the means compared through Tukey test at 5% of significance, using ASSISTAT software version 7.7.

RESULTS AND DISCUSSION
There was a significant interaction effect between P application and soil density on corn root and shoot P content (Table 1).Root P content at density 1 did not show significant difference among P application, whereas at density 2 higher root P content was observed on plants treated with in line P compared to broadcast and without P (Table 2).There was no effect of soil density on root P content when in line P application was used; however, higher root P content was observed at the lowest density in plants under broadcast P application and without P treatment (Table 2).These results support the hypotheses that soil compaction decrease P diffusion flow, thus reduces ion-root contact and plant uptake.According to Barbosa et al. (2018) the lower levels of P provided by broadcast fertilization P are justified by the low initial level of this nutrient in the soil, causing a reduction in the availability of P within the reach of the root.Amanullah and Almas (2012) affirmed the application of localized P enhances root system and provides upper  contact with nutrients.

Density
At density 1, in line application of P resulted in higher shoot P content compared to broadcast and without P application (Table 2).In contrast, at density 2 higher shoot P content was observed in plants under in line and broadcast application compared to plants without P fertilization (Table 2).Regardless of soil density effect within P application, no significant effect was observed in plants without P fertilization.However, density 1 resulted in higher shoot P content when P was broadcast and in line applied compared to those without P application.This might be explained by the dilution effect (Martuscello et al., 2009), which is elucidated by the higher dry matter content of corn plants showed on Table 3.
Accumulation of P in shoots and roots was higher with in line P application than with broadcast and without P (Table 1).It was observed that in line P increased 37 and 24% of P accumulation in roots and shoots, respectively, compared to broadcast application.Souza et al. (2016) verified in line application of phosphate fertilizers may increase concentration of P in the zone of initial radicular growth compared to broadcast application.Da Ros et al. ( 2017) verified that for in plants of corn and sunflower, the location of the phosphate fertilizer is very important in the accumulation of P in the plants.Moreover, in no till areas, broadcast application of P enhances P content in the superficial soil layer compared to sub superficial layers (Santos et al., 2005) which increases contact between phosphate fertilizer and soil.Therefore, higher rate is required in broadcast application to achieve similar fertilizer efficiency than in line application (Prado et al., 2001).
There was higher accumulation of P in shoot and root of plants cultivated in soil with density 1 than in soil with density 2 (Table 1).Therefore, it was noticed that soil density of 1.6 g cm -3 was harmful to root and shoot growth and P uptake.Also, soil compaction results in higher resistance to mechanical penetration that limits radicular development and reduces root capacity of water uptake, hence it affects plant growth (Ortigara et al., 2014).
Plants with P applied in line and broadcast were 94.83% and 84.70%, respectively, which are higher than plants without P fertilization (Table 3).This is explained by P essentiality on plant metabolism, the component of nucleotides used in energy metabolism of plants (such as ATP) and in DNA and RNA (Taiz et al., 2017); resulting in smaller plants in soils with lower P availability.
A significant interaction effect between density and P application was observed for the other measured variables (Table 3).At density 1, stem diameter was higher with application of P in line than broadcast fertilization and without P (Table 4).In contrast, similar diameter was observed with in line and broadcast application at density 2, which were higher than those without P application.There was no effect of soil density on plant diameter when P was not applied and applied in line; however, higher stem diameter was observed at density 2 when broadcast was applied.
Regardless of soil density, root volume was higher when P was applied in line compared to broadcast application and without P application (Table 4).Moreover, higher root volume was observed at density 1 when P was in line applied.Since P has low mobility in soil, lower root volume affects uptake of P by corn plants (Santos et al., 2005) and also affects root distribution and biomass within soil profile ( (Twum and Nii-Annang, 2015); resulting in reduction in crop yield.This fact elucidates the importance of knowing crop threshold for soil compaction, as well as of monitoring soil compaction, especially in tropical regions in which there is low levels of available P.
Regardless of soil density, root and shoot dry matter accumulation were higher in plants under in line P application, expect for root dry matter in plants broadcast applied with P, in which similar results to in line application were observed (Table 4).
Moreover, plants treated with in line P presented lower accumulation of root and shoot dry matter at soil density 2. The effect of soil density in plants applied with broadcast P was only observed for root dry matter, in which lower values was observed at density 2. No effect of soil density on root and shoot dry matter was observed in plants without P application.Grzesiak et al. (2014) observed the negative effect of soil compaction in maize and triticale, with a decrease of aerial parts and roots dry matter and changes in roots distribution in the soil profile.Sarto et al. (2018) Table 4. Interaction effect between P application method and soil density on stem diameter (SD), root volume (RV), root dry matter (RDM) and shoot dry matter (SDM) of corn plants cultivated in greenhouse condition.elucidated that in compacted soil, the roots under hypoxia produce high quantity of ethylene.This hormone inhibits root elongation and induces root swelling in plants (Geisler-Lee et al. 2010).This might explain higher root values (60%) in plants applied with in line P at soil density 1.2 g cm -3 (D1) compared to soil density 1.6 g cm -3 (D2).Grzesiak et al. (2013) indicate that soil compaction reduces plant system roots; consequently, affecting the development and productivity of several crops (Gubiani et al., 2014;Valadão et al., 2015) Shoot dry matter of plants that received in line P application was 60% higher than broadcast application, and 11 times higher than plants without P fertilization (Table 4).According to Santos et al. (2005), maximum accumulation of shoot dry matter in corn plants cultivated in pots with nutritive solution occurred with application of 400 mg dm -3 of P (NH 4 H 2 PO 4 ).These authors observed a maximum of 6.02 g pot -1

Density
, which was lower than shoot dry matter observed in the present study (11 g pot -1 ). Souza et al. (2008) also observed that application of 400 mg dm -3 of P in corn plants cultivated at densities of 1.34 a 1.67 g cm -3 resulted in 4.5 a 3.5 g of shoot dry matter, respectively; these were also lower than shoot dry matter accumulation observed for plants treated with in line P in the present study.This might be due to the application method used, in which corn dry matter increases with increasing availability of P through localized application of fertilizers, as observed in the present study with in line treatment.
Therefore, higher root and shoot dry matter (Table 4) might be explained through higher accumulation of P in corn root and shoot (Table 1).These results elucidate the importance of accurate management of phosphate fertilization in order to increase plant nutrition.

Conclusions
Phosphorus application in line resulted in higher P accumulation in plant.There was higher plant dry matter accumulation at soil density 1.2 g cm -3 compared to 1.6 g cm -3 . In line phosphate fertilization at soil density 1.2 g cm -3 enhances dry matter accumulation in corn plants.

CONFLICT OF INTERESTS
The author has not declared any conflict of interest.
both column and row of the same superscript are not significantly different (p<0.05).
column and row of the same superscript are not significantly different (p<0.05).

Table 1 .
Effect of P application method and soil density on root P content (RPC), shoot P content (SPC), root P accumulation (RPA) and shoot P accumulation (SPA) of corn plants cultivated under greenhouse condition.
* and ** of F test significant at p<0.01 and p<0.05, respectively.

Table 2 .
Effect of interaction between P application method and soil density on root P content and shoot P content of corn plants cultivated under greenhouse condition.

Table 3 .
Effect of P application method and soil density on stem diameter (SD), plant height (PH), root volume (RV), root dry matter (RDM) and shoot dry matter (SDM) of corn plants cultivated in greenhouse condition.
** of F test significant at p<0.01, respectively.