Study on termite damage to different species of tree seedlings in the Central Rift Valley of Ethiopia

A high density of termite mounds is common in the Maki-Batu area of the Central Rift Valley of Ethiopia. To determine whether farmers consider termites as one of their problems in growi ng trees and practices they use to manage termites, short semi-structured questionnaires were distributed to farmers selected randomly. To evaluate the efficacy of some non-chemical control methods, Eucalyptus camaldulensis seedlings of five months were transplanted from nursery beds at two experimental sites. The seedlings were planted in three blocks each containing 6 treatments with 3 replicates laid out in randomized complete block design (RCBD). The treatments were: wood ash, cow dung, maize stalk, wood ash + cow dung + maize stalk, Untreated/control and Diazinon 60% EC applied at 2 l/ha as spray on the soil surface and a soil pit drench as a standard check. Plant mortality assessment was recorded once every month for one year. Mortality data were analyzed by analysis of variance (ANOVA) with SAS at 5% probability level. To find out the impact of termites on tree seedlings after transplanting in the area, mortality assessment was conducted at five sites on 11 species of tree seedlings transplanted from nursery beds. Termite damage on the seedlings was recorded once every month. After 12 months, seedlings of each plant species damaged during each month were summed and results were computed as percentages. To assess farmers’ perceptions of termites as pests, unstructured questionnaires were distributed to 64 farmers randomly selected. Eucalyptus seedling mortality was very low and there was no significant difference among the treatments (P > 0.05) except at one site in which wood ash + manure + maize stover showed higher mortality. Seventy-eight percent of the farmers mentioned that they had planted tree seedlings for different purposes and 72% of them considered termites as the major cause of seedling mortality. Although, higher density of Macroterems termite mounds were found in the area and most farmers considered termites as one of the major constraints in growing tree seedlings, this study showed very low termite damage to the tree seedlings studied for one year after transplanting.


INTRODUCTION
Termites by no means confine their attentions to dead plant tissues such as wood.Certain species of termites are serious pests of growing crops including living trees (Hickin, 1971) and they are one of the major agroforestry pests in the tropics (Nyeko and Olubayo, 2005).Many other insect pest species cause damage to various parts of the tree, but often they do not cause mortality (Logan et al., 1990;UNEP, 2000).However, termites are seldom primary pests, only damaging the plant, shrub or tree when it has already been affected by fire, disease, drought, mechanical injury, bad planting or other insect pests (Harris, 1971;Hickin, 1971).Although, termites usually appear to be secondary pests, it does not make them of less importance.The initial defect affecting the plant is often of minor importance, after allowing ingress to termites, the effect may be the complete destruction of the plant or, at least, a reduction in its value as a crop (Hickin, 1971).
The extent to which termites are problems to trees and the nature of loss they cause are very much related to the geographic region concerned (Logan et al., 1990).In the tropical and sub-tropical regions of the world where rainfall is low and a dry savannah-type of situation has developed, termite attacks appear most acute and this has caused serious problems in the development of nurseries and young tree plantations.Another phase of the problem is the susceptibility to termite of a popular group of tree species for the tropical planting, the Eucalyptus (Hickin, 1971).Eucalyptus is preferred as compared to other plants such as Cassia, Albizzia and Gmelina species because of its more rapid growth potential (Harris, 1971).Eighteen termite species are recorded as damaging young trees in forest and plantation nurseries and there are a number of recorded instances of high percentage loss, even complete and total destruction of young trees, particularly Eucalyptus (Hickin, 1971).Moreover, Pearce (1997) noted that a high demand for timber in Africa has led to fast-growing trees such as Eucalyptus, being planted in poor soil areas where they are under stress and are therefore more susceptible to termites.
The main obstacle to afforestation in the dry areas of Africa is said to be the presence of termites (Harris, 1971;Hickin, 1971).However, not all species of termites present in a locality are destructive to forestry (Nair, 2007).The species ranking as forestry pests vary in different tropical areas.In Africa, the genera, Macrotermes and Odontotermes are implicated as well as the species Pseudacanthotermes militaris in Uganda.Throughout tropical Africa, losses are due exclusively to members of the subfamily: Macrotermitinae, all of which are fungus growers with large nests in mounds or underground (Harris, 1971;Hickin, 1971).
As elsewhere in Africa, termite damage in Ethiopia is generally greater in rain-fed than irrigated crops, during dry periods or drought than periods of regular rainfall, in plants under stress (e.g.newly transplanted forest tree seedlings) than healthy or vigorous plants and exotic (e.g.Eucalyptus, maize) than indigenous plants (Wood, 1986(Wood, , 1991)).In western Ethiopia, termite damage on indigenous trees is insignificant.In contrast, serious damage is very common on exotic forestry trees, especially on Eucalyptus, one to three years after transplanting (Abdurahman, 1995).Soon after transplanting, seedlings suffer severely from moisture stress, because of soil compaction and low water holding capacity resulting from poor infiltration rates.The roots of such plants begin to dry out and this creates a favorable situation for termite infestation (Gauchan et al., 1998).In some localities up to 100% of Eucalyptus seedling loss is common (Abdurahman, 1995).In most areas of Uganda, an average of between 30 and 70% of planted Eucalyptus trees are killed by termites (Mazodze, 1995).
Control of termites as forest pests involves both chemical and traditional methods.Current chemical control methods employed are soil treatment, treatment of seedlings before transplanting and baiting techniques and the chemicals currently used include chlorpyrifos, imidacloprid and fipronil.In addition to the current chemical control methods employed, there are a number of alternative, traditional control methods, largely relating to silvicultural practices or plantation management, which are also very important, and should be considered before chemical intervention is attempted.Many traditional methods of control of termites in forest plantations have a sound basis in the principles of ecology (UNEP 2000).Logan et al. (1990), UNEP (2000) and Abdurahman et al. (2010) have reviewed a wide range of control of termites in crops and forestry with non-chemical methods which include cultural and biological control.
Though a high density of termite mounds was reported in the Maki-Batu (Batu formerly called Ziway) area of the Central Rift Valley of Ethiopia (CRVE), no data were available on the extent of termite damage on forestry tree seedlings after transplanting.Therefore, the main objectives of the study were to assess the impact of termites on some species of tree seedlings and test the efficacy of some non-chemical control methods on Eucalyptus seedlings after transplanting.

Study area
The study w as conducted betw een July 2012 and January 2014 in tw o districts (Bora and Adami Tullu-Jiddo Kombolcha) of East Shaw a Zone of Oromia Regional State at six sites (Figure 1).The districts are located in the Central Rift Valley of Ethiopia.Maki is the capital of Dugda district and Batu is the capital of Adami Tulu Jido Kombolcha district.The capital tow ns of the tw o districts are about 30 km from each other along Addis Ababa -Shashemene high w ay.
In the context of this study, Maki-Batu area therefore includes the tw o districts and neighboring districts w here high density of *Corresponding author.E-mail: getahundan@yahoo.com,daniel.debelo@astu.edu.Tel: +251 911 38 46 59.Author(s) agree that this article remain permanently open access under the terms of the Creativ e Commons Attribution License 4.0 International License Macrotermes termites are found.
The area has a semi-arid and arid climate and has a bimodal rainfall patterns (Mengistu, 2008).The highest precipitation occurs betw een July and September w ith some additional rainfall betw een February and the end of April, but this usually varies.Rain-fed crops are most often not planted until mid-June because of the unpredictable rainfall pattern in the preceding period (Haimanot, 2002).Coordinates, elevations, mean annual rainfall and temperatures of the capital tow ns of the three districts are show n in Table 1.
The vegetation in the CRVE is characterized by acacia openw oodland and savanna (Huib and Herco, 2006).The natural vegetation of the area is under high pressure due to the expansion of cultivated land, overgrazing and deforestation and thus the natural flora and fauna are disappearing rapidly (Huib and Herco, 2006;Mengistu, 2008).

Farmers' constraints in growing tree seedlings and termite management practices
Short semi-structured questionnaires w as dispatched to 64 randomly selected smallholder farmers focusing on general problems they faced in grow ing tree seedlings (to assess w hether they consider termites as one of the problems) and practices they used to manage termites in tree seedlings.Farmers' responses w ere analyzed and reported as percentages.
To evaluate the efficacy of some non-chemical control methods

Eucalyptus seedlings m ortality under different control options
E. camaldulensis seedlings of five months old w ere planted in three blocks each containing six plots (2.5 x 2.5 m).Thirty -six seedlings w ere planted per plot (648 seedlings per site) w ith a spacing of 0.5 x 0.5 m and 1 m distance betw een blocks w ith 3 replications laid out in randomized complete block design (RCBD) based on Mazodze (1995).A total of six treatments w ere used: T1 (w ood ash), T2 (cow dung), T3 (maize stalk), T4 (w ood ash + cow dung + maize stalk), T5 (untreated/control) and T6 (Diazinon 60% EC) applied at 2 l/ha as spray on the soil surface and as a soil pit drench around the seedlings as a standard check.Six maize stalks of 40 cm length w ere buried 20 cm deep in the soil around each seedling in the treated plots; 0.5 kg of cow dung and 0.5 kg of w ood ash w ere added around the base of the treated seedlings.The Eucalyptus seedlings raised in plastic bags on nursery beds w ere provided by Agricultural and Rural Development Bureau of Adami Tulu Jido Kombolcha district and transplanted to the site located at GWPS on July 14, 2012 and to WWKAFTC research site on July 16, 2012.
Eucalyptus seedlings mortality data w ere analyzed by analysis of variance (ANOVA) w ith SAS (SAS Institute, 2003).Significant differences betw een treatments w ere compared by the least significant difference (LSD) test at 5% probability level.Before analysis data w ere square root transformed (X + 0.5) 1/2 to stabilize heterogeneity of variation (Gomez and Gomez, 1984).

Mortality of different species of tree seedlings due to term ites
Eleven different species of tree seedlings w ere transplanted from nursery beds at five sites.Eucalyptus seedlings w ere planted by the researcher on three farmers' fields, w hile Sesbania sesban seedlings w ere planted on one farmer's field.The tree seedlings at Garbi Widana Boramo Kebele Administration Farmers Training Center (GWBKAFTC) site w ere planted by the w orkers of the Farmers Training Center (FTC) and those at Meles Green Park site w ere planted by the local community.
All the seedlings w ere raised in plastic bags on nursery beds and w ere provided freely by Agricultural and Agricultural and Rural Development Bureau of Adami Tulu Jido Kombolcha district.The seedlings w ere transported to sites by vehicles w ith care.
Termite damage on the seedlings w as recorded starting one month after transplanting and continued every month for 12 months.After 12 months, the numbers of plant dried due to termite infestation of each plant species and site w ere summed and results w ere computed as percentages.
During each assessment, w ilted and/or dried plants w ere checked for signs of termite attack above and below the ground.Causes of plant mortality w ere ascertained by lifting the dried plant and examining the root and stem for typical Macrotermes and Microtermes damage.The former species cut the base of w ellestablished plants, w hile the latter species enter and consume the larger roots and prop-roots and continue their excavation into the stem, w hich can be excavated and packed w ith soil (Maniania, 2002).Termite species that w ere found attacking the saplings w ere collected and preserved in 80% ethanol.Taxonomic identification w as done at the genus level using Keys to the Genera of Ethiopian Termites based on soldier characters (Abdurahman, 1991).
Plant mortality assessment w as started on the first month after planting, and continued once a month for one year.The numbers of plant that dried due to termite infestation of each plant species and site w ere summed and results w ere computed as percentages.

RESULTS
Seventy-eight percent of the respondent farmers mentioned that they had planted tree seedlings for different purposes such as house construction, shade, and for creating good air condition, beauty and environmental rehabilitation.Farmers mentioned that they had faced certain constraints which hinder the establishment of the seedlings after transplanting and their further growth.Of those farmers that planted tree seedlings, 72% considered termites as the most serious problems causing tree seedling mortality followed by livestock (browsing), mole rats and drought (Table 2).

Farmers' termite management practices
Forty percent of those respondent farmers who mentioned termites as problems in growing tree seedlings used different methods of indigenous control, mainly a combination of two or more methods which include good silviculture, cow dung, wood ash and queen removal (Table 3).No one used chemical control method (synthetic termiticide).Application of cow dung and wood ash around base of plants were the major practices used by farmers.Respondents mentioned that termites do not appear in areas where decomposing manure is found.

Eucalyptus seedling mortality under different control options
Seedling mortality due to termites under the different treatments is shown in Table 4. Seedling mortality due to termites under all the treatments was very low.However, higher mortality was recorded in plots treated with combined wood ash, manure and maize stover at GWPS site.There was no significant difference among the treatments at WWKAFTC site.

Mortality of different species of tree seedlings due to termites
The mean percent mortality of different species of tree seedlings due to termites is shown in Table 5.No mortality was recorded in most of the species of the tree seedlings.However, a relatively higher mortality (6.6%) was recorded only on Eucalyptus at FF 3 .Damage was caused mainly by Macrotermes and to less extent by Odontotermes and Microtermes.

DISCUSSION
Most of the respondent farmers considered termites as one of the major problems in growing tree seedlings and this is basically in agreement with Sileshi et al. (2008) who reported similar results consistent with evidence from literature.For instance, Ugandan farmers ranked termites as the most serious problems in growing trees.However, damage of termites on the tree seedlings, even on the most susceptible exotic plant, Eucalyptus, was very low and this was contrary to a wide range of literatures (Hickin, 1971;Harris, 1971;Abdurahman, 1990;Pearce, 1997) and farmers' report in this study.
High demand for timber in Africa has led to fast-growing trees, such as Eucalyptus, being planted in poor soil areas where they are under stress and are therefore more susceptible to termites (Pearce, 1997).In Cameroon, 100% losses were recorded with Eucalyptus saligna, and 60 to 80% with other species; in the drier areas of Uganda between 50 and 70% of transplants were regularly lost; in Northern Nigeria, E. camaldulensis had a failure rate of between 68 and 74% in the first eighteen months and 86% after thirty months (Harris , 1971).Cowie and Wood (1989) reported that in some areas in southern, western and eastern Ethiopia, some forest trees are seriously damaged and attack is usually on newly-transplanted seedlings, particularly Eucalyptus, and losses could exceed 90%.Further, Abdurahman (1995) noted that in western Wallaga (Ethiopia), termite damage on indigenous trees is insignificant but serious damage is very common on exotic forestry trees, especially on Eucalyptus, one to three years after transplanting.In some localities up to 100% of Eucalyptus seedling loss is common (Abdurahman, 1995).As per the personal communication made with experts of Asosa Plant Health Clinic, Asosa Zone-, Mana Sibu-and Ayira-districts of Agricultural and Rural Development Offices, termites ranked as number one of all the existing pests.They claimed that there would be total losses in Eucalyptus unless chemical termiticides are used.The critical period to prevent termite attack in young plantations of tree seedlings is during the first year in the nursery and a few months after planting out.Later on, as the trees get older, termite infestation may increase, but once a good canopy is formed, attack is often greatly reduced.A positive correlation exists between the presence of drought conditions and incidence of attack (Pearce, 1997).
Tree species like Acacia senegal, Acacia albida, Cordia africana, Ficus vista and Olea africana are all indigenous and the absence of damage be attributed to this factor.Pearce (1997) noted that some of the common tree species seen today in some regions have resulted from selection by termites, that is, the more susceptible ones have been eaten to extinction many years ago.Many of these indigenous trees are therefore more resistant to termite attack and have developed chemical defenses to protect themselves.
The absence of damage or the incident of little damage recorded both on indigenous and exotic tree seedlings may also be attributed to the absence of pest species, low termite population density, and presence of amble food.Abdurahman et al. (2010)  All the study sites contained amble wood and grass litter and these might provide sufficient food for termites and thus diverted termites' attention from attacking the tree seedlings.In western Wallaga, where termite damage on Eucalyptus seedlings is very high, farmers weed the seedlings and remove the debris out of the fields which leave termites with little food and thus force them to attack the seedlings.
On the other hand, significantly higher termite damage in the plots of Eucalyptus seedlings treated with combined wood ash, manure and maize stover than the other treatments at GWPS site might be attributed to the attraction of termites to manure and maize stover.Animal dung and wood are ideal foods for Macrotermes termites.In the present study, all the termite species recorded in the area were sampled by using maize stover used as baiting than the other treatments Logan et al. (1990) reported that there are conflicting views on the use of crop residues and other organic matter in termite control.The addition or removal of organic matter from the field has been suggested as methods of reducing levels of attack.Essentially, the conflicting principles are (1) the removal of residues and other debris from the field will reduce potential termite food supplies and hence lead to reduction in termite numbers and subsequent attack; and (ii) that leaving residues in the field or adding further organic matter will provide alternative food to which the termites will be attracted, thereby reducing levels of attack.The respective counter-arguments are (i) that removal of alternative food sources will concentrate termite activity on the crops/trees, leading to increased damage; (ii) that leaving residues and other food sources in the field will provide extra resources and allow termite populations to build up, again to increase damage.There is evidence supporting both views and a generalization may not be possible, different methods being appropriate in different places with different termite species and different crops/trees.The authors have also reported that the use of wood ash is a common practice which demands proper evaluation.
For damage to crops and trees, the first sign of termite presence, especially in seedlings, is wilting.Also, soil runways or sheeting on the soil surface, plants or trees indicates that termites are present.Termites can be collected from the runways and identified.To check for the presence of termites in the field, samples of unhealthy looking plants from the crop should be taken (Pearce, 1997).In the current study, mostly termites of the genus Macrotermes were found damaging tree seedlings.Termites of the genus Macrotermes are the most common termites in the Central Rift Valley of Ethiopia responsible for lodging of maize plants which cut the plants at ground surface (Daniel and Emana, 2015).Rural houses in the area are mostly damaged by Macrotermes (Emana and Daniel, 2014).The genus Macrotermes includes several important pests of a wide range of field crops and trees.They attack plants by cutting off young seedlings or mature plants at soil level (Nyeko and Olubayo, 2005).

Conclusion
Although, higher density of Macroterms termite mounds are found in the area and most farmers considered termites as one of the major constraints in growing tree seedlings, this study showed very low termite damage to the tree seedlings.The reason for the low damage may be that the termite population is low and/or the termites had enough other food options to eat and thus avoided attacking the seedlings.

Figure 1 .
Figure 1.Map show ing East Shaw a Zone of Oromia Regional State (Source: Daniel and Emana, 2015).

Table 1 .
Coordinates, elevations, mean annual rainfall and temperature at the meteorological stations located in the capital tow ns of the three districts.
a.s.l.).Kebele is the low est administrative level w hich is below district.

Table 2 .
Problems mentioned by farmers in grow ing tree seedlings after transplanting at Central Rift Valley of Ethiopia.

Table 3 .
Percentage of respondent farmers that used different indigenous termite management practices against tree seedlings in the Central Rift Valley of Ethiopia.
*The percentages do not sum up to100 because some individuals mentioned that they used more than one management options.

Table 4 .
Eucalyptus seedlings mortality (mean ± SE) one year after transplanting to WWKAFTC site and GWPS site in the Central Rift Valley of Ethiopia.

Table 5 .
Mean percent mortality of tree seedlings due to termites after transplanting to different sites in the Central Rift Valley of Ethiopia.