Valorization of Jatropha curcas seed cake into different kinds of compost and effect of these composts on cabbage yield in Togo

Jatropha curcas L. is a plant species from the family of Euphorbiaceae. The extraction of oil from its seeds produces a cake for which its accumulation causes different environmental problems related to solid waste management. The valorization of this cake into compost could be a contribution to solving this problem. Therefore different types of compost were made with J. curcas seed cake associated with animal feces incorporated at 0, 10 and 20% rate. To achieve their combination, 5 composting test and 2 replicates each have been set up. These composts were tested on cabbage yield in a completely randomized design with 6 treatments and 3 replicates. The different composts have improved significantly the chemical characteristics of the soil and the cabbage biomass yield. Mixing J. curcas seed cake with 10% sheep’s droppings (C10) gave chemical characteristics compost with a high N and K content (Total-N 18.32 g.kg -1 ; Total-K 17.60 g.kg -1 ). The compost made with J. curcas seed cake and 20% sheep’s droppings (C20) had the best cabbage total biomass and shoots of cabbage biomass yields respectively 43.11±3.15×10 3 and 21.56±1.58×10 3 kg.ha -1 . That compost C20 could be recommended to farmers for the improvement of crop yield field after trials followed by validation.


INTRODUCTION
Jatropha curcas L. is a drought-resistant shrub belonging to the family of Euphorbiaceae. It can live up to 50 years and grow on poor soils (Henning, 2008). This plant is widespread in all arid and semi-arid tropical areas of the world. It becomes an important industrial crop due to its seed which contains 30 to 40% oil with properties similar *Corresponding author. E-mail: sogbedjikomi@webmail.hzau.edu.cn.
Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License to those of fossil diesel (Sanou, 2010). J. curcas can easily be propagated by cuttings. It is widely planted as a hedge to protect fields because of the fact that it is not browsed by cattle or other animals. It is often used to protect soil from erosion (Martinez-Herrera et al., 2006;Samake, 2007). The seeds of J. curcas are used in medicines, soap and cosmetics manufacture in various tropical countries (Domergue and Pirot, 2008). To obtain biodiesel from J. curcas it is necessary to extract the oil from its seeds. After this extraction, the obtained byproduct is the seed cake. This seed cake can be used for biogas production and composts production (Domergue and Pirot, 2008;Raheman and Mondal, 2012). Although the seed cake is rich in protein (Staubmann et al., 1997), it is toxic to rats, mice and ruminants and therefore cannot be used as an animal forage nor can it be used directly as fertilizer in agricultural farming due to the potential presence in the seed cake of toxic elements that are saponin, curcin and phorbol esters (Akintayo, 2004;Gübitz et al., 1999).
In Togo, more than 80600 ha would be reserved for the production of J. curcas (Demba, 2011) to produce great quantity of biofuel. Some companies began already to exploit J. curcas in Togo. Approximately 15150 tons of J. curcas seed cake is produced each year (Demba, 2011). These seed cakes accumulation causes different environmental problems related to solid waste management. Previous research indicated that a huge volume of J. curcas seed cake can be eliminated through composting or by biogas production (Das et al., 2011).
However, the decrease of soil fertility is of a particular concern for both farmers and researchers in Togo. Famers face high cost of mineral fertilizers and scarcity of manure. Researchers are looking for new technologies to maintain or restore fertility of degraded soils. Extensive operating system of land based on shifting cultivation and long-term fallow had the advantage of being balanced and allowed to maintain a relatively stable agricultural production (Zoumana and César, 1994). Today, the above mentioned balance is broken due to the population growth that is leading to more changes in land use at the expense of fallow and forests. Therefore, to improve soil fertility using J. curcas seed cake, this study was conducted aiming to: (1) assess the compost made with J. curcas seed cake alone or J. curcas seed cake associated with animal feces (sheep's droppings or chicken dejection) and (2) assess the effectiveness of these composts in improving soil chemical properties and cabbage shoot yield.

MATERIALS AND METHODS
The study was conducted at the University of Lomé Research Station in Lomé, Togo (6°10'N, 1°10'E; altitude = 19-60 m). The soil type was a rhodic ferralsol locally called "Terres de Barre" that developed from a continental deposit (Saragoni et al., 1991). This soil is red, deep and suitable for almost all crops. The chemical characteristics of the soil of the site before trial were: Organic-C= Sogbedji et al. 4669 7.60 g.kg -1 , Total-N = 0.90 g.kg -1 , Total-P= 0.024 g.kg -1 , Total-K= 0.115 g.kg -1 . These characteristics show that the levels of N, P and K are relatively low rendering this soil as a suitable experimental site. The experiment was done at ambient temperature. The experiment site's climate is sub-equatorial type with 2 rainy seasons and 2 dry seasons where the average annual rainfall is less than 1100 mm and the average annual temperature is 27°C. The following materials were used to develop different types of compost submitted to agronomic test: J. curcas seed cake which is the main source of organic material used was procured by PISA IMPEX TOGO (Agro-Industry in Togo: Production of palm kernel oil, soya oil, Neem oil and biofuel with J. curcas seed); the animal feces used as fermentative bacteria sources were sheep's droppings and chicken dejection from the School of Agronomy Research Station of Lomé. They were incorporated at the rate of 10 and 20% in the heap.
The effect of different types of compost was evaluated on Oxylus variety of cabbage (Brassica oleracea L.) whose heads could weigh about 2.5 or 3 kg. The cabbage was chosen because of its easy handling and rapid growth that allow to set a controlled cropping area and to speed up data collection as well as its demanding for N, P, and K nutrients. The experiment was conducted in 2 phases: production of different composts and agronomic test Composting was carried out in compost bins with cemented bottoms to prevent effluents from the compost pile to seep into the soil. The piles were covered with plastic sheeting which protects the compost from the air and reduces the release of heat generated inside the pile. This method of composting is an aerobic method that combines Indore and Bangalore methods with modifications (Toundou et al., 2014). It differs from the Indore method by the lack of brush at the base of the heap (compost bin bottom was cemented). However, it is closed to the Bangalore method because the compost bin is completely covered by plastic sheeting (instead of mud or soil's clods use in Bangalore method) protecting ambient air and preventing the release of heat generated inside the pile.
For the preparation of compost, 2 factors were involved: the nature of the incorporated animal manure (sheep's droppings or chicken dejection) and the rate of incorporation of animal manure (0, 10 and 20% of the heap mass) deriving in the following combinations: C0, C10, C20, F0, F10 and F20. Animal feces are adjuvant to the heap and their rates are randomly selected. To achieve their combination, 5 composting test were replicated 2 times each in a total of 10 experimental units that have been designed instead of 6 composting test. J. curcas seed cake compost objects without the addition of animal feces C0 and F0 are identical to Tm which is the control (Table 1).
Before planting and at harvesting, experimental soils samples were collected from of 0 to 15 cm soil depth to test pH-H2O, pH-KCl, Total N, Total P, Total K and organic carbon. These analyses were done in the Laboratory of Soil and Plants Analyses of the Superior School of Agronomy and in the Water Chemistry Laboratory, Faculty of Science, University of Lomé. Total N was determined by Kjeldahl method, digestion in a mixture of H2SO4-selenium followed by distillation and titration (Bremner and Mulvaney, 1982); total P by (Murphy and Riley, 1962) method, K by (Gueguen and Rombauts, 1961) method; organic carbon (Walkley and Black, 1934), wet oxidation with K2Cr2O7 procedure; pH-H2O and pH-KCl using glass  The harvest took place on the 76th day after transplanting. Using a knife, the plants were cut just below the basal leaves. The cabbages were harvested and weighed using scales CAMRY to determine fresh biomass yield.
Statistical analyses were performed using STATISTICA. The parameters were subjected to a one way analysis of variance. Duncan's multiple range test (DMRT) was performed to compare differences in means among treatments. All significance levels were set at P<0.05.

Source of nutrient
Control (T0) Tm C10 C20 F10 F20 Biomass (t.ha-1) chicken dejection in J. curcas seed cake before composting seems to increase the pH of the compost. On the other hand, the pH of the compost C 20 or F 20 is less than the ones containing 10%. The later had the highest alkalinity (pH = 8.27). The mixture of J. curcas seed cake with 10% of sheep's droppings produced good quality compost with the highest levels of N (18.32 g.kg -1 ) and K (17.60 g.kg -1 ). Figure 2 shows the effect of different types of compost used on biomass of cabbage shoots. All tested composts produced a cabbage shoot biomass which was significantly higher than the control. The C 20 had the highest biomass significantly higher than the rest. This was followed by the C 10 treatment. The highest yield is obtained with compost C 20 , C 10 and F 20 composts have acted in the same way on performance. The lowest yields were obtained from the T m compost even though this was not statistically different from the F 10 and F 20 treatments.

Effect of different composts used on the total biomass of cabbage heads
The effect of different types of used compost in the total biomass of cabbage is presented in Figure 3. All tested composts had a significant increase in the total biomass of cabbage heads when compared with the control. Compost C 20 had the best performance in total biomass whereas composts T m , F 10 and F 20 had lower cabbage head biomass.

Soil chemical properties after harvesting cabbage as influenced by different nutrient sources
The soil chemical characteristics (mean values of pH-H 2 O, pH-KCl, Total N, Total P, Total K and organic carbon) after harvesting were presented in Table 3. Compost C 10 and C 20 had a slight increase in soil alkalinity. The contents of carbon and N decreased on the soil before trial compared to the soil on the plot without compost (T 0 ). All composts made increased the soil P content as compared with that before trial.
statistically identical. Therefore, adding sheep's droppings had no effect on N content. Unlike the incorporation of chicken dejection reduced the total N content. The high levels of K and P contents in F 20 compost could be attributed to the chicken dejection. Adding animal feces might be improve J. curcas seed cake compost in K and P contents. The incorporation rate seems to have no effect on the C:N ratio. The C:N ratios obtained ranging from 8.72 to 12.35 are favorable for microbiological processes in the soil (Busby et al., 2007). However C:N ratio cannot be used as an absolute indicator of compost quality because of the large variability in raw materials and often gives a misleading indication of maturity (Das et al., 2011). A wide range of C:N is mentioned in the literature for composts, example for compost in fermentation phase, the C:N ratio ranges from 10 to 80 (Bernal et al., 1998;Koledzi, 2011). This ratio decreases during composting to reach values ranges from 8 to 25, which is explained by the fact that microorganisms consume more C than N (Bernal et al., 1998;Koledzi, 2011).
The positive effect of different composts on the production of shoots of cabbage was observed in Benin with composts of municipal organic waste improved with chicken dejection and maize stover residues (Amadji, 2006;Amadji et al., 2009). These results show that various composts produced by J. curcas seed cake (T m , C 10 , C 20 , F 10 and F 20 ) have increased significant effect on the cabbage shoot yield. Compost made with J. curcas seed cake can be used as fertilizer to improve culture yield.
Different composts increased the pH of the soil. That could be due to cations present in the various composts (T m , C 10 , C 20 , F 10 and F 20 ) at the maturity (Weber et al., 2007). This increase of the pH value after using various composts was observed on hydromorphic soil under cabbage (Fassinou, 1996), on ferralitique soil under radish and amaranth (Cédric, 1997) and on a sandy soil under cabbage (Amadji, 2006).
The soil content of C and N decreased in the soil control compost (T 0 ). These increased in the soils with different kinds of compost namely T m , C 10 , C 20 , F 10 and F 20 . This clearly shows that the addition of different composts has raised C and N contents in soil. The control (T m ) and the F 20 compost were the most improved soil in C and N content.
Generally, different composts made by J. curcas seed cake have improved the chemical characteristics of the soil. It is still to know the effects of phytotoxicity spreading at high doses and for long time use of different composts made with J. curcas seed cake on crops and soil. Future research will take into account this aspect. It will be good to extend this test to determine the equivalent of mineral fertilizers from composts prepared.

Conclusion
The C 10 compost produced good chemical characteristics compost with a high N and K content (Total-N 18.32 g.kg -1 ; Total-K 17.60 g.kg -1 ). The C 20 compost produced the highest head and shoot biomass respectively 43.11±3.15 10 3 kg.ha -1 and 21.56±1.58 10 3 kg.ha -1 . The different composts made with J. curcas seed cake improved significantly the chemical characteristics of the soil. The incorporation of sheep's droppings has improved the chemical characteristics of the J. curcas seed cake compost, even though adding chicken dejection would not improve J. curcas seed cake compost maybe due to their composition. Sheep's droppings richer than chicken dejection would be due to sheep's alimentation. However, the applicability and potential use of such compost as a soil amendment should be assessed effectively through field trials followed by validation prior to recommendation.
improved the quality of the first draft of this paper.