African Journal of
Agricultural Research

  • Abbreviation: Afr. J. Agric. Res.
  • Language: English
  • ISSN: 1991-637X
  • DOI: 10.5897/AJAR
  • Start Year: 2006
  • Published Articles: 6671

Full Length Research Paper

Supplementation of selenium and vitamin E in diets for pacu (Piaractus mesopotamicus): Effect on performance, body yields and lipid stability

Elenice Souza dos Reis Goes
  • Elenice Souza dos Reis Goes
  • Post Graduate in Animal Science, Universidade Estadual do Oeste do Paraná, Rua Pernambuco, 1777, Marechal Candido Rondon, Paraná, 85960-000 Brazil,
  • Google Scholar
Aldi Feiden
  • Aldi Feiden
  • Faculdade Street, Universidade Estadual do Oeste do Paraná, Rua da Faculdade 645, Toledo, Paraná Brazil.
  • Google Scholar
Wilson Rogério Boscolo
  • Wilson Rogério Boscolo
  • Faculdade Street, Universidade Estadual do Oeste do Paraná, Rua da Faculdade 645, Toledo, Paraná Brazil.
  • Google Scholar
Marcio Douglas Goes
  • Marcio Douglas Goes
  • Post Graduate in Aquaculture and Sustainable Development, Universidade Federal do Paraná, Rua Pioneiro, 2153, Palotina, Paraná 85950-000 Brazil.
  • Google Scholar
Altevir Signor
  • Altevir Signor
  • Faculdade Street, Universidade Estadual do Oeste do Paraná, Rua da Faculdade 645, Toledo, Paraná Brazil.
  • Google Scholar


  •  Received: 14 August 2015
  •  Accepted: 15 October 2015
  •  Published: 14 January 2016

 ABSTRACT

Current study evaluates the supplementation of selenium and vitamin E in the diet on performance, body yields and lipid stability of pacu. Seven hundred and twenty juveniles were distributed in 36 cages installed in a masonry tank. During 100 days, the animals were fed on diets supplemented with four selenium levels (0, 1, 2 and 4 mg/kg), combined with three levels of vitamin E (0, 100 and 200 mg/kg) in a 4x3 factorial arrangement. Selenium combined with vitamin E levels did not affect (P>0.05) growth and body yield of pacu. The interaction between levels of selenium and vitamin E did not influence (P>0.05) the lipid stability of main trunks. When the levels of vitamin E only are evaluated, 200 mg vitamin E/kg diet decreases lipid oxidation in 90 and 120 days of storage. The combination of 0 Se + 0 vitamin E / kg diet was influenced by storage time, and the highest rate of lipid oxidation was observed with 120 days of storage. Level 1 mg selenium + 200 mg vitamin E/kg in the diet of the pacu decreased meat lipid oxidation after 90 days of storage under freezing, coupled to the maintenance of performance and body yields.

Key words: Aquaculture, dietary antioxidants, lipid oxidation.


 INTRODUCTION

In Brazilian aquaculture, the pacu Piaractus mesopotamicus (Holmberg, 1887) stands out for its high growth rate, easy adaptation to aquaculture systems and high fecundity (Sampaio et al., 2008), coupled to other factors such as its meat quality, with good acceptance by consumers (Jomori et al., 2003). Small quantities of vitamins and minerals are required for the animals normal growth, reproduction, health and metabolism (Lovell, 1998). Vitamin E is the most important metabolic antioxidant in the cell membrane, protecting it from oxidation by fatty acids and cholesterol, and reducing or inhibiting the production and action of free radicals
 
 
(Sampaio et al., 2004). Besides being a component of several selenoproteins, Selenium is a co-factor and an integral part of the glutathione peroxidase enzyme (GPx) (Rotruck et al., 1973).
 
Vitamin E is included in the non-enzymatic antioxidant system of the animal organism, while selenium, as member of GPx, integrates the enzymatic system for antioxidant protection. Selenium and vitamin E act in synergy, since selenium cannot protect the cell or tissue components that have low GPx concentrations (Rotruck et al., 1973). However, these cell or tissue components may be protected by vitamin E, which acts as antioxidant by different mechanisms. Combined selenium and vitamin E are the main antioxidants in the organism.
 
Antioxidants from the diet do not have only important functions in living organisms but they may decrease the lipid oxidation in tissues after death. Studies on vitamin E supplementation in diets for fish revealed its antioxidant action in in vivo and in the reduction of oxidation after death, with an improvement in conservation during storage (Onibi et al., 1996; Pirini et al. 2000; Shiau and Shiau, 2001; Ruff et al., 2002; Hamre et al., 2004; Fogaça and Santana, 2007).
 
However, the literature reports no studies involving the relationship between selenium and vitamin E for pacu, both in performance and lipid oxidation after death. Current analysis evaluates the antioxidant effects of selenium and vitamin E supplementation in the diet on the pacu´s productive performance, body parameters and lipid stability.


 MATERIALS AND METHODS

The experiment was conducted at the Institute of Research in Environmental Aquaculture (InPAA), Universidade Estadual do Oeste do Paraná (UNIOESTE), Toledo campus, Toledo PR Brazil, from March to July 2011, for 100 days. Seven hundred and twenty pacu juveniles (P. mesopotamicus), retrieved from a commercial fish farm, with initial average weight = 43.52±1.03 g and total length = 12.33±1.65 cm, were used in current assay. Fish were distributed in 36 cages and installed in a 200m² masonry tank. The 1 m³ cages were made ​​of polyester coated with flexible PVC and with a 5 mm mesh. The experimental unit was a tank with 20 juveniles. Fish were kept in the experimental structures for seven days previously to adapt themselves to the experimental conditions.
The experiment comprised twelve treatments with three replicates per diet. The experimental design was completely randomized, in a 4x3 factorial arrangement, with four selenium inclusion levels (0, 1, 2 and 4 mg / kg) and three vitamin E inclusion levels (0, 100 and 200 mg/kg diet).
 
The experimental diets were formulated to obtain 26% crude protein and 3000 kcal digestible energy/kg (Table 1), where composition of feed was calculated with software SuperCrac (SUPERCRAC, 2004). DL-α-tocopherol was the additional source of vitamin E, with activity of 50% vitamin  E,  while  sodium  selenite with 45% selenium availability was employed for selenium supplementation.
 
The ingredients for the processing of rations were initially ground in a hammer-type mill with a 0.5 mm sieve; they were then milled, weighed and mixed for the preparation of the experimental diets. Different levels of vitamin and mineral supplements were added to the mixture. The mixtures were humidified (28% water) and extruded through a EX-MICRO® mill with 10 kg/h production capacity. The diets were dried in a forced-air oven for 12 h, at 55°C, resulting in a product with approximately 10% moisture. Feeding was carried out twice a day, at 10:00 am and 5:00 pm, by apparent satiation and the amount of diet provided was weighed to estimate apparent feed conversion.
 
The water tank temperature was measured daily at 10:00 am and 5:00 pm and the water physical and chemical parameters (pH, dissolved oxygen and electrical conductivity) were measured once a week at 6:00 am and 4:00 pm with portable meters (YSI Model 55 Dissolved oxygen, YSI Incorporated, Yellow Springs, USA and Alkafit pHmeter AT315 SP, Alkafit, Florianópolis, Brazil). At the end of the experiment, fish were fasted for 24 h to empty the digestive tract. The fish were then removed from the experimental units and anesthetized in benzocaine at 250mg /l (euthanasia), packed in ice inside a cooler and transported to the Fish Technology Laboratory (UNIOESTE), campus Toledo, Toledo PR Brazil.
 
Individual measures of final weight, total and standard lengths were taken for weight gain calculation (WG = final weight - initial weight), apparent feed conversion (FCR = feed intake / weight gain) and fish survival in each experimental unit. Afterwards, the animals were then opened at the ventral abdominal cavity, from the urogenital hole up to the jaw bones, followed by careful viscera removal to prevent contamination of meat with fecal material. The head was cut and the fish, without head and viscera, were washed in chlorinated water, removing fins and skin, leaving only the trunk. Intraperitoneal fat and liver were manually removed from the viscera, and weighed. Data was used to calculate the hepatosomatic index [HSI = (liver weight / fish weight) x (100)] and intraperitoneal fat index [IGI = (intraperitoneal fat weight / fish weight) x (100)].
 
The main trunks were placed in plastic bags and stored in a freezer (18±2°C) for 30 days, when chemical analyzes were performed (moisture, protein, lipids and ash), according to methodology proposed by AOAC (2005).
 
Lipid oxidation was analyzed by the thiobarbituric acid reactive substances method (TBARS) (Vyncke, 1970) to investigate the lipid stability of meat after 60, 90 and 120 days of storage. The amount of malondialdehyde (MDA), the main substance formed during the oxidation and which reacts with thiobarbituric acid, was calculated by the standard curve equation: y = 73.689 + 0.0223 x (r ² = 0.9968). Results were expressed as mg MDA per kg of sample.
 
Data underwent analysis of variance (ANOVA) at 5% significance in a factorial arrangement, verifying the interaction between selenium and vitamin E. When significant interaction was reported, Duncan test´s was applied at 5% significance to compare means. Data were also evaluated for homogeneity of variances (Levene´s test) with statistic program SAEG 9.1 (UFV, 2007).


 RESULTS AND DISCUSSION

Water temperature in the tanks averaged 20.8±2.62°C during the experiment. There was a linear temperature decrease throughout the experimental days (y = -0.074 + 23.291 x r ² = 0.7325) since the experiment began in March and ended in July (period of low temperatures). The physical and chemical parameters of the tank water were 4.67±0.75 mg O2D · L-1; 7.26±0.64 and  40.08±2.63 mS cm-1 for dissolved oxygen, pH and electrical conductivity, respectively. Selenium and vitamin E levels did not affect (P>0.05) the parameters total and standard length, weight gain, feed conversion and survival (Table 2).
 
There was no significant interaction (P> 0.05) between selenium and vitamin E on performance and survival, and selenium and vitamin E separately did not affect productive performance.
 
Similar results in current study were reported for tilapia (Oreochromis niloticus) fed on different levels of organic selenium (0, 0.25, 0.50, 1.0, and 1.5 mg Se / kg) in the diet, with no significant difference in performance (weight gain and apparent feed conversion) (Gomes, 2008). Further, in a study on juvenile Sparus aurata evaluating the effect of supplementation of 250 mg vitamin E/kg diet in a system of high stocking density (40 kg/m3), Montero et al. (1999) did not report any effect (P> 0.05) on weight gain and survival. There was no difference in growth for the Atlantic halibut (Hippoglossus hippoglossus) between treatments with 189 and 613 mg vitamin E / kg in the diet (Ruff et al., 2002).
 
Current study confirms results by Sampaio (2003) who evaluated levels of selenium (0, 0.25, 0.50 and 1.00 mg / kg diet) and vitamin E (0,100, 200 and 300 mg / kg) for the tilapia (O. niloticus). The author failed to register any effect of the interaction of these nutrients on weight gain, feed conversion and survival rate. Studying largemouth bass (Micropterus salmoide) fed on graded levels of vitamin E (160, 280, and 400 mg/kg) associated with either 1.2 or 1.8 mg/kg selenium (Se), Chen et al. (2013) reported that vitamin E and selenium inclusion could protect largemouth bass from the oxidative damage challenged by dietary oil oxidation, although none could enhance growth and feed utilization.
 
The absence of any significant effect of diets on production performance differs from results by Cavichiolo et al. (2002), who evaluated the effect of vitamins C and E on Nile tilapia larvae (O. niloticus). They reported that treatment with 300 mg vitamin E per kg diet provided increased weight and final length of larvae, and reduced the occurrence of the ectoparasite Trichodina sp in tilapia larvae. In the case of hybrid tilapia (O. niloticus x O. aureus) fed on increasing levels of vitamin E, Huang and Huang (2004) found greater weight gain in tilapia fed on 62.5 IU vitamin E / kg diet. Likewise, Gonçalves et al. (2010) registered that supplementation with 400 mg / kg vitamin E improved the standard length and weight gain of tambacus (Colossoma macropomum × P. mesopotamicus).
 
Rainbow-trout (Oncorhynchus mykiss) fed on 50 ppm de vitamin E and on 0.35 ppm selenium improved gain weight and food conversion. There was also an improvement of the fillet´s functional quality when compared to control (Rodríguez and Rojas, 2014).
 
The highest selenium level in current experiment (4 mg/kg diet) did not cause any damage on fish performance. In a study with Pogonichthys macrolepidotus juveniles fed on 0.4, 0.7, 1.4, 2.7, 6.6, 12.6, 26.0 and 57.6 mg selenomethionine / kg diet levels, Teh et al. (2004) observed a histopathologic effect only when concentrations were greater than or equal to 6.6 mg Se / kg diet. The selenium poisoning occurred in the rainbow-trout when diets contained selenium levels exceeding 13 mg / kg (Hilton et al., 1980). Thus, the level of 4 mg selenium / kg diet for pacu (P. mesopotamicus) does not seem to cause poisoning and deterioration in fish performance. However, Lin and Shiau (2005) emphasized that the minimum selenium level required by fish diet differed according to the species, or rather, between 0.25 and 0.80 mg / kg.
 
Although selenium supplementation in the diet of pacu did not cause any damage on fish performance, the concentration of 1.5 mg Se / kg diet improved growth and increased   antioxidant   defenses   of   matrinxã   (Brycon cephalus) by increasing the activity of the enzymes glutathione peroxidase and increased the level of reduced glutathione (Monteiro et al., 2007). Trunk yield, intraperitoneal fat index and hepatosomatic index of pacu fed on diets with different selenium and vitamin E levels did not differ significantly (P>0.05) when treatments were assessed (Table 3). Moreover, selenium and vitamin E separately did not affect (P>0.05) main trunk yields, hepatosomatic index and intraperitoneal fat.
 
 
Signor et al. (2010) reported approximately a 60% main trunk yield, or rather, above the average value of 50.60% found in current analysis. An increase in fish size provided a higher main trunk yield.
 
The intraperitoneal fat index was lower than that reported by Bittencourt et al. (2010) and Signor et al. (2010), although similar to that observed by Hilbig et al.,(2012) for the pacu. Change in the intraperitoneal fat index occurred  if  fish  required  fat  to  obtain  energy,  a fact that occurred during long fasting (Hilbig et al., 2012). However, since this experiment had an apparent satiation food supply, the fish probably did not need to mobilize lipids for energy production.
 
Despite the lack of significant difference (P> 0.05) in the hepatosomatic index of pacu fed on different levels of selenium and vitamin E, fish with selenium accumulation in tissues tended to have a higher hepatosomatic index (Pyle et al. 2005). However, the latter was not observed in this experiment, since the hepatosomatic index was equal for the minimum selenium level and for a greater inclusion of dietary selenium. Checking the above, Gomes (2008), who evaluating levels of organic selenium (0, 0.25, 0.50, 1.0, and 1.5 mg Se/kg) in the diet of tilapia (O. niloticus), reported no significant difference in the hepatosomatic index. For P. mesopotamicus with average weight of 377g, Hilbig et al. (2012) found a 0.77% average  hepatosomatic  index, a lower  rate  than 
that  in that in current assay, which averaged 2.75%.
 
Moisture, protein, lipids and ash contents of pacus were not significantly different (P>0.05) among supple-mentations with selenium and vitamin E (Table 4), neither was there any difference when selenium and vitamin E levels were evaluated separately. In fact, results corroborated those by Otani (2009) who did not register any significant difference in moisture, protein and ash contents in fillets of tilapia fed on 0 and 100 mg α-tocopherol per kg diet. However, the same author observed increased lipid contents in these fillets when compared to diet without antioxidant addition, or rather, the use of antioxidants in the diet may protect the lipids of fillets from lipid oxidation, during freezing.
 
In a study evaluating supplementation with organic selenium  (0.0, 0.25, 0.50, 0.75 and 1.0 mg / kg) in the diet of Nile tilapia matrices, Pereira et al. (2009) did not report any significant differences in moisture, protein  and lipid contents of fish fed on different selenium levels. Results corroborated those in current study.
 
Current assay revealed there was no difference in the proximate composition of pacu for different levels of vitamin E. However, Sau et al. (2004) obtained significant differences (P<0.05) in protein rates of rohu carcass (Labeo rohita) at different levels of vitamin E supple-mentation in the diets. In this study, carcasses of the group fed on diets with 200 mg / kg had lower average percentage of crude protein (57.17%) when compared to groups with 100 mg/kg (58.34%) and 0 mg/kg (58.34%). There were no differences in proximate composition with means 70.9 and 71.2% moisture, 9.7 and 9.5% lipids, 18.4% crude protein and 1.5% ash, respectively, for groups 300 and 1500 mg / kg, in rainbow-trout fed on 300 and 1500 mg vitamin E/kg diet (Chaiyapechara et al., 2003). Results of lipid oxidation for the main trunks of pacus fed on different levels of selenium and vitamin E are shown in Table 5.
 
 
 
In the case of lipid oxidation in different evaluation times, only level 0 Se + 0 vit. E / kg diet  was  affected  by storage time, and the highest oxidation rate was observed for 120 days. However, when selenium levels were evaluated separately, levels 0 and 4 of Se / kg diet were significantly influenced by storage time, and the lipid oxidation was higher after 120 days (P<0.05) when compared to that after 60 and 90 days. Vitamin E supplementations (0, 100 and 200 mg vit. E / kg diet) were also affected by different storage periods, with significant increase (P<0.05) of TBARS overtime, as shown in Table 5.
 
This observation was also made by Otani (2009) for tilapia fillets and by Weber et al. (2008) for silver catfish fillets, perhaps due to an increase of compounds formed by lipid oxidation which reacts with thiobarbituric acid, thereby increasing TBARS rates. However, these rates ​​tended to decrease overtime since there was a reduction of the substrates which reacted with thiobarbituric acid, as reported by Otani (2009).
 
The combination of selenium and vitamin E did not affect (P>0.05) lipid oxidation of fillets, neither did selenium levels separately affect this parameter. 
 
 
However, when the levels of vitamin E were assessed separately (Figure 1), levels of vitamin E influenced lipid oxidation after 90 and 120 days of storage and the level of 200 mg vitamin E / kg diet provided a lower rate (P<0.05). This finding showed the postmortem anti-oxidant effect of adding vitamin E on the diet of pacu (P. mesopotamicus). Cheah et al. (1995) claim that vitamin E is effective in fish conservation during processing and storage, since it inhibits the degradation of lipids by oxidation.
 
The antioxidant properties of vitamin E were also observed by Huang and Huang (2004), who found that dietary supplementation of vitamin E for hybrid tilapia (O. niloticus x O. aureus) decreases lipid peroxidation of postmortem tissue, whereas increase of dietary vitamin E causes decrease in muscle TBARS. The above authors observed that tilapia fed on 300 IU vitamin E / kg diet had the lowest TBARS rates ​​in the muscle:  1.47 nmol  MDA / mg. Moreover, Huang and Huang (2004) also found that vitamin E supplementation increased the glutathione level in the liver.
 
This is probably due to the contents of vitamin E stored in the muscle, which are greater in fish fed on higher doses of vitamin E. Vitamin E is actually a potent biological antioxidant: its high levels in tissues inhibit lipid peroxidation and decrease the formation of malondialdehyde. This trend has also been reported in the Atlantic salmon (Onibi et al., 1996) and sea bass (Gatta et al., 2000).
 
Results similar to current study were provided by Fogaça and Santana (2007) with tilapia fed on 0, 100 and 200 vit.E mg / kg diet and after 63 days processed in burgers. The authors noted that the burgers in the control group (without the addition of vitamin E) had higher TBARS rates ​​than treatments with vitamin E supplemen-tation. In fact, vit.E 200 mg / kg was the best  to decrease to decrease lipid oxidation since it indicated Vitamin E´s antioxidant activity.
 
 
Thus, the addition of 200 mg vitamin E / kg diet in pacus is ideal to reduce lipid oxidation after 90 and 120 days of storage, under freezing. This is due to the fact that vitamin E supplementation in the diet makes it incor-porated into lipid membranes, protecting the tissue from any post-mortem oxidation.
 
Level 1 mg selenium + 200 mg vitamin E / kg in the diet of juvenile pacu (P. mesopotamicus) decreases lipid oxidation of meat after 90 days of storage, under freezing, and maintains performance and body yields.


 CONFLICT OF INTERESTS

The authors have not declared any conflict of interests.



 REFERENCES

Abimorad EG, Carneiro DJ (2004). Métodos de coleta de fezes e determinação dos coeficientes de digestibilidade da fração protéica e da energia de alimentos para o pacu (Piaractus mesopotamicus) Holmberg, 1887. R. Bras. Zootech. 33(5):1101-1109.
Crossref

 

AOAC – Association of Official Analytical Chemistry. (2005). Official Methods of Analysis of the AOAC. 18. Ed. Gaithersburg, M.D, USA.

 
 

Bittencourt F, Feiden A, Signor AA, Boscolo WR, Maluf MLF (2010). Densidade de estocagem e parâmetros eritrocitários de pacus criados em tanques-rede. R. Bras. Zootec. 39(11):2323-2329.
Crossref

 
 

Cavichiolo F, Ribeiro RP, Moreira HLM, Loures BRR, Maehana K, Povh JA, Leonardo JMLO (2002). Efeito da suplementação de vitamina C e vitamina E na dieta, sobre a ocorrência de ectoparasitas, desempenho e sobrevivência em larvas de tilápia do Nilo (Oreochromis niloticus L.) durante a reversão sexual. Acta Sci. Anim. Sci. 24(4):943-948.

 
 

Chaiyapechara S, Casten MT, Hardy RW, Dong FM (2003). Fish performance, fillet characteristics, and health assessment index of rainbow trout (Oncorhynchus mykiss) fed diets containing adequate and high concentrations of lipid and vitamin E. Aquaculture 219(1-4):715738.
Crossref

 
 

Cheah KS, Cheah AM, Krausgrill DI (1995). Effect of dietary supplementation of vitamin E on pig meat quality. Meat Sci. 39(2):255264.
Crossref

 
 

Chen YJ, Liu YJ, Tian LX, Niu J, Liang GY, Yang HJ, Yuan Y, Zhang YQ (2013). Effect of dietary vitamin E and selenium supplementation on growth, body composition, and antioxidant defense mechanism in juvenile largemouth bass (Micropterus salmoide) fed oxidized fish oil. Fish Physiol. Biochem. 39(3):593-604.
Crossref

 
 

Fogaça FHS, Sant’Ana LS (2007). Tocopherol in the lipid stabilitity of tilapia (Oreochromis niloticus) hamburgers. Food Chem. 105(3):1214-1218.
Crossref

 
 

Gatta PP, Pirini M, Testi S, Vignola G, Monetti PG (2000). The influence of different levels of dietary vitamin E on sea bass, Dicentrarchus labrax flesh quality. Aquacult. Nutr. 6(1):47-52.
Crossref

 
 

Gomes GR (2008). Suplementação com selênio orgânico nas dietas de tilápias do nilo (Oreochromis niloticus). Master's dissertation. Universidade Estadual Paulista, Centro de Aquicultura, Jaboticabal, Brazil.

 
 

Gonçalves ACS, Murgas LDS, Rosa PV, Navarro RD, Costa DV Teixeira EA (2010). Desempenho produtivo de tambacus alimentados com dietas suplementadas com vitamina E. Pesq. Agropec. Bras. 45(9):1005-1011.
Crossref

 
 

Hamre K, Christiansen R, Waagbo R, Maage A, Torstensen BE, Lygren B, Lie O, Wathne E, Albrektsen S (2004). Antioxidant vitamins, minerals and lipid levels in diets for Atlantic Salmon (Salmo salar L.): effects on growth performance and fillet quality. Aquacult. Nutr. 10(2):113-123.
Crossref

 
 

Hilbig CC, Boscolo WR, Feiden A, Dieterich F, Lorenz EK Zaminhan M (2012). Feeding rate for pacu reared in net cage. R. Bras. Zootec. 41(7):1570-1575.
Crossref

 
 

Hilton JW, Hodson PV, Slinger SJ (1980). The requirement and toxicity of selenium in rainbow trout (Salmo gairdneri). J. Nutr. 110(12):2527-2535.
Pubmed

 
 

Huang CH, Huang SL (2004). Effect of dietary vitamin E on growth, tissue lipid peroxidation, and liver glutathione level of juvenile hybrid tilapia, Oreochromis niloticus × O. aureus, fed oxidized oil.

 
 

Jomori R, Carneiro DJ, Portella MC (2003). Growth and survival of pacu Piaractus mesopotamicus (Holmberg, 1887) juveniles reared in ponds or at different initial larviculture periods indoors. Aquaculture 221(1-4):277-287.
Crossref

 
 

Lin YH, Shiau SY (2005). Dietary selenium requirements of juvenile grouper, Epinephelus malabaricus. Aquaculture 250(1-2):356-363.
Crossref

 
 

Lovell RT (1998). Nutrition and feeding of fish. 2. ed. Massachusetts: Kluwer Academic Publishers.
Crossref

 
 

Montero D, Marrero M, Izquierdo MS, Robaina L, Vergara JM, Tort (1999). Effect of vitamin E and C dietary supplementation on some immune parameters of gilthead seabream (Sparus aurata) juveniles subjected to crowding stress. Aquaculture 171(3-4):269-278.
Crossref

 
 

Monteiro DA, Rantin FT, Kalinin AL (2007). Uso do selênio na dieta de matrinxã, Brycon cephalus. Rev. Bras. Saúde Prod. Anim. 8(1):32-47.

 
 

Onibi GE, Scaife JR, Fletcher TC, Houlihan DF (1996). Influence of αtocopherol acetate in high lipid diets on quality of refrigerated Atlantic Salmon (Salmo salar) fillets. Sci. Technique Froid 33:145152.

 
 

Otani FS (2009). Influência da adição in vivo de vitamina E e de métodos de abate nos atributos de qualidade de filés de tilápia. Master's dissertation. Centro de Aquicultura da Unesp - Universidade Estadual Paulista, Jaboticabal, São Paulo, Brazil.

 
 

Pereira TS, Fabregat TEHP, Fernandes JBK, Boscolo CN, Castillo JDA, Koberstein TCRD (2009). Selênio orgânico na alimentação de matrizes de tilápia-do-Nilo (Oreochromis niloticus). Acta Sci. Anim. Sci. 31(4):433-437.
Crossref

 
 

Pirini M, Gatta PP, Testi S, Trigari G, Monetti PG (2000). Effect of refrigerated storage on muscle lipid quality of sea bass (Dicentrarchus labrax) fed on diets containing different levels of vitamin E. Food Chem. 68(3):289293.
Crossref

 
 

Pyle GG, Rajotte JW, Couture P (2005). Effects of industrial metals on wild fish populations along a metal contamination gradient. Ecotoxicol. Environ. Saf. 61(3):287-312.
Crossref

 
 

Rodríguez PH, Rojas MS (2014). Efecto de dietas enriquecidas con vitamina e y selenio orgánico en el comportamiento productivo y calidad funcional del filete de trucha arco iris (Oncorhynchus mykiss). Rev. Inv. Vet. Perú 25(2):213-225.

 
 

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973). Selenium: biochemical role as a component of glutathione peroxidase. Sci. 179(4073):588-590.
Crossref

 
 

Ruff N, Fitzgerald RD, Cross TF, Teurtrie G, Kerry JP (2002). Slaughtering method and dietary tocopherol acetate supplementation affect rigor mortis and fillet shelf-life of turbot (Scophthalmus maximus). Aquacult. Res. 33(9):703-714.
Crossref

 
 

Sampaio FG (2003). Selênio e vitamina E em dietas para tilápia do Nilo (Oreochromis niloticus). Master's dissertation. Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.

 
 

Sampaio FG, Kleemann GK, Sá MVC, Pereira ASP, Barros MM, Pezzato LE (2004). Níveis de vitamina E e de selênio para pós-larvas de Macrobrachium amazonicum. Acta Sci. Anim. Sci. 26(1):129-135.

 
 

Sampaio FG, Boijink CL, Oba ET, Santos LRB, Kalinin AL, Rantin FT (2008). Antioxidant defenses and biochemical changes in pacu (Piaractus mesopotamicus) in response to single and combined copper and hypoxia exposure. Comp. Biochem. Physiol. C Toxicol. Pharmacol. Part C 147(1):43-51.

 
 

Sau SK, Paul BN, Mohanta KN, Mohanty SN (2004). Dietary vitamin E requirement, fish performance and carcass composition of rohu (Labeo rohita) fry. Aquac. 240(1-4):359368.
Crossref

 
 

Signor AA, Boscolo WR, Feiden A, Bittencourt F, Coldebella A, Reidel A (2010). Proteína e energia na alimentação de pacus criados em tanques-rede. R. Bras. Zootec. 39(11):2336-2341.
Crossref

 
 

Shiau SY, Shiau LF (2001). Reevaluation of the vitamin E requirement of juvenile Tilapia (Oreochromis niloticus x O. aureus). Anim. Sci. 72:529534.

 
 

SUPER CRAC (2004). Ração de custo mínimo. Versão 1.02 para Windows. [S.I]: TD Software.

 
 

Teh SJ, Deng X, Deng DF, Teh FC, Hung SSO, Fan TWM, Liu J, Higashi RM (2004). Chronic effects of dietary selenium on juvenile Sacramento split-tail (Pogonichthys macrolepidotus). Environ. Sci. Technol. 38(22):6085-6093.
Crossref

 
 

Universidade Federal de Viçosa (UFV) (2007). SAEG 9.1 Sistema para analises estatísticas genéticas, Viçosa: MG.

 
 

Vyncke W (1970). Direct determination of the thiobarbituric acid value in trichloracetic acid extracts of fish as a measure of oxidative rancidity. Fette. Seifen. Anstrichm. 72(12):1084-1087.
Crossref

 
 

Weber J, Bochi VC, Ribeiro CP, Victório AM, Emanuelli T (2008). Effect of different cooking methods on the oxidation, proximate and fatty acid composition of silver catfish (Rhamdia quelen) fillets. Food Chem. 106(1):140-146.
Crossref

 

 




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