Soil born plant pathogen cause potential yield loss in every year all over world. Antifungal Trichoderma isolates can control soil borne plant pathogenic fungi. Soil sample collected from different location to find out effective Trichoderma isolates. Their antifungal and morphological characteristic was studied. Radial mycelial growths of the isolates varied from 29.91 to 90.00 mm in vitro assay. On the basis of shape, growth habit, colony color, compactness, Spore density isolates were categorized in different group. Different selected temperature such as: 20o, 25 o, 30o and 35o C were maintained in incubators to observe the growth habit of different Trichoderma isolates. Optimum growth of Trichoderma isolates was recorded twenty 25 to 30 ºC. Antifungal activities of all Trichoderma isolates were placed against fungal mycoflora viz. Penicillium sp, Fusarium oxysporum, Sclerotium rolfsii, Rhizoctonia solani, Aspergillus flavus, Colletotrichum gloeosporiodes and Phomopsis vexans by duel culture method. All isolates of Trichoderma showed wide variation in growth inhibition against these pathogens. Among all of the Trichoderma isolates, ST5 was strongly inhibited the growth of all the pathogenic fungi.

Keywords: Trichoderma spp, Antifungal, Bio-control agent, Soil-borne fungus.