African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12237

Full Length Research Paper

Study of genetic polymorphism of Artemisia herba-alba from Tunisia using ISSR markers

Haouari Mohsen* and Ferchichi Ali
Laboratoire d'Aridoculture et Culture Oasienne, Institut des Régions Arides 4119 Medenine- Tunisia.
Email: [email protected]

  •  Accepted: 20 August 2007
  •  Published: 04 January 2008


Artemisia herba-alba is an herbaceous aromatic and therapeutic plant widely distributed in semi-arid regions of Tunisia and is potentially usable to restore degraded ecosystems. A study of genetic variation among 216 accessions was conducted using ISSR (Inter Simple Sequence Repeat) markers to assess the polymorphism at the species level. A total of 60 polymorphic loci were scored using four primers revealing a high level of genetic polymorphism among A. herba-alba accessions. Correlation analysis revealed no direct relation between morphological traits, geographic distance and genetic distance. Correlogram analysis showed a patchy distribution of the genetic variability of A. herba-alba accessions revealing the contribution of local ecological and geographic conditions on variability.


Key words: Artemisia herba-alba, DNA extraction, genetic polymorphism, ISSR markers.