African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12465

Full Length Research Paper

Stimulation of growth and some biochemical parameters by magnetic field in wheat (Triticum aestivum L.) tissue cultures

Sema Alikamanoglu and Ayse Sen*
Department of Biology, Faculty of Science, Istanbul University, 34459, Vezneciler, Istanbul, Turkey.
Email: [email protected]

  •  Accepted: 08 August 2011
  •  Published: 14 September 2011



In this study, various magnetic field intensities between 2.9 to 4.8 mT by 1ms-1speed applied to mature embryos of Flamura-85 wheat variety and effects of magnetic field on different physiological (regeneration rate, average plant fresh weights, and average plant lengths) and biochemical [total protein, and chlorophyll amounts, superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX) enzyme activities] parameters were investigated. Compared to the control group, an increase was detected on physiological parameters of 2.2 and 19.8 s magnetic field applied Flamura-85 wheat variety. Total protein and chlorophyll amounts, SOD, CAT, POX and APX enzyme activities of 2.2 and 19.8 s magnetic field applied experimental groups presented increase compared to the control, depending on applied magnetic field intensity.


Key words: Antioxidant enzymes, chlorophyll amount, growth parameters, magnetic field, plant tissue culture, protein amount, wheat.


AL, average plant length;  2,4-D, 2,4-dichlorophenoxyacetic acid;EDTA, ethylenediaminetetraacetic acid; fw, average plant fresh weight; MF,magnetic field; MS, Murashige and Skoog (1962); NBT,  nitro-blue tetrazolium; SD,standard deviation; SOD, superoxide dismutase; CAT, catalase; POX, peroxidase;APX, ascorbate peroxidase.