African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Lipid lowering and anti-atherosclerotic properties of Tinospora crispa aqueous extract on high-cholesterol diet-induced hyperlipidemic rabbits

Zamree, M. S.
  • Zamree, M. S.
  • Innovation and Commercialization Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Ihsan Safwan, K.
  • Ihsan Safwan, K.
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Khairul Kamilah A. K.
  • Khairul Kamilah A. K.
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Mohd Kamal N. H.
  • Mohd Kamal N. H.
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Rasadah, M. A.
  • Rasadah, M. A.
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Mohd Shahidan M. A.
  • Mohd Shahidan M. A.
  • Innovation and Commercialization Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Daryl, J. A.
  • Daryl, J. A.
  • Faculty of Health Sciences, Universiti Teknologi Mara (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
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Zulkhairi, A.*
  • Zulkhairi, A.*
  • Malaysian Nuclear Agency (MINT), Bangi, 43000 Kajang, Selangor, Malaysia.
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  •  Received: 10 June 2015
  •  Accepted: 20 August 2015
  •  Published: 26 August 2015

Abstract

This study was aimed to investigate the hypocholestrolemic and anti-atherosclerotic properties of Tinospora crispa aqueous extract (TCAE) on rabbits for 10 weeks. The hyperlipidemic rabbits were induced and the rabbit were given different concentration of TCAE (200, 450 and 600 mg/kg). Results from lipid analysis show that the level of total cholesterol (TC), triglyceride (TG) and LDL-C on the hyperlipidemic rabbits were reduced with the treatment of TCAE while HDL level was elevated. Through plasma analysis, the activity of gamma glutamyl transferase (GGT) and alkaline phosphates (ALP) were also reduced with the treatment of TCAE compared to hyperlipidemia group. All group of rabbits tested with TCAE again had significantly higher (p < 0.05) total antioxidant status (TAS), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities. Among the concentrations of TCAE tested, medium dose showed more potent effect in reducing blood serum TC, TG and LDL-C levels and increasing HDL-C level compared to low and high dosages counterparts. No foam cell formation was visible in aorta of rabbits treated with TCAE in dose dependent manner. However, there was visible foam cell formation in the aorta of hyperlipidemia group. In conclusion, this study suggests that supplementation of 450 mg/kg of T. crispa extract would be able to reduce or retard the progression of atherosclerotic plaque development induced by dietary cholesterol.

 

Key words: Hypocholesterolemia, Tinospora crispa, anti-atherosclerotic properties, cardiovascular diseases.

Abbreviation

TCAE, Tinospora crispa aqueous extract; GGT, gamma glutamyl transferase; ALP, alkaline phosphates; TAS, total antioxidant status; GPx, glutathione peroxidase; SOD, superoxide dismutase; CVD, cardiovascular diseases; TC, total cholesterol; TG, triglyceride; HDL-c, high density lipoprotein-cholesterol; LDL-c, low density lipoprotein-cholesterol; GSH-Px, glutathione peroxidase; VLDL, very low density lipoprotein; IDL, intermediate density cholesterol; ROS, reactive oxygen species.