African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Biological control of Aspergillus flavus growth and subsequent aflatoxin B1 production in sorghum grains

K. R. N. Reddy1*, C. R. Raghavender2, B. N. Reddy2 and B. Salleh1
1School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia, 2Department of Botany, Osmania University, Hyderabad, India.
Email: [email protected]

  •  Accepted: 07 April 2010
  •  Published: 31 July 2010

Abstract

Sorghum grains suffer from severe infection and colonization by several toxigenic fungi and subsequent production of mycotoxins, posing a threat to human and animal health. Among all the mycotoxins, aflatoxins represent one of the most important toxicants considered as important constraint of grain quality and sorghum production. Various physical and chemical methods of reducing mycotoxins have been recommended, but only few have been accepted for practical use. Biodegradation of aflatoxins, deploying microbes is an attractive strategy for mycotoxin management. This study, therefore explored the potential use of certain biocontrol agents for the reduction of growth of toxigenic Aspergillus flavus and subsequent aflatoxin B(AFB1) production in sorghum. Among all the biocontrol agents tested, culture filtrate of Rhodococcus erythropolis completely inhibited theA. flavus growth and AFB1 production at 25 mlkg-1 concentration. The other biocontrol agents, Bacillus subtilis, Pseudomonas fluorescens and Trichoderma viride showed 72, 74 and 65% inhibition of A. flavus growth whereas 54, 62.6 and 39% reduction of AFB1 at 200 mlkg-1 of sorghum grains, respectively.

 

Key words: Sorghum, Aspergillus flavus, AFB1, biological control.

Abbreviation

AFB1, Aflatoxin B1ELISA, enzyme-linked immunosorbent assay;IgG-ALP, immunoglobulin G -alkaline phosphatase; BSA, bovine serum  albumin; PBST, phosphate buffered saline tween-20.