African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

The role of bone marrow derived mesenchymal stem cells in induced stroke

Intissar Numman Waheed*
  • Intissar Numman Waheed*
  • Department of Biology, Faculty of Science, Zakho University, Duhok, Iraq.
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Anwar Abdul-Ameer Mohammed Karim
  • Anwar Abdul-Ameer Mohammed Karim
  • Department of Biology, College of Science, Diyla University, Iraq.
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Mohammad Abdul-Hadi Gali
  • Mohammad Abdul-Hadi Gali
  • Department of Biology, College of Science for Women, Baghdad University, Baghdad, Iraq.
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  •  Received: 22 February 2014
  •  Accepted: 29 October 2014
  •  Published: 19 November 2014

Abstract

Stroke is the third most common cause of death, and a leading cause of physical disability in adults. Recovery after a major stroke is usually limited, but cell therapy, especially by application of mesenchymal stem cells (MSCs) is emerging with fixed neurologic deficits. The aim of the current study was directed to isolation and cultivation of the bone marrow (BM) derived MSCs from young rats, as well as to study the role of intravenous administration of BM-MSCs in mature male rats as an animal model for Middle Cerebral Artery Occlusion (MCAO). MSCs are spindle in shape fibroblast-like cells and possess the ability to aggregate and form colonies-forming unit – fibroblast (CFU-F). MSCs showed positive response for CD105+ (the specific marker for MSCs detection) and negative response for surface marker (CD34¯), characteristic for the hematopoietic cells. The immunohistochemistry study of intravenous administration of Bromodeoxyyuridin (BrdU) labeled BM-MSCs after 24 h of mechanical MCAO in mature rats, demonstrated survival, engraftment and migration of systemically delivered cells in the cerebral cortex and heart tissues. However, these cells were not indicated in the lung and liver tissues. In conclusion, intravenously administered BM-MSCs enter brain and heart, and survive of this, may provide a cell source to treat stroke and heart disease. 
 
Key words: Middle cerebral artery occlusion, mesenchymal stem cells, rats, transplantation, bromodeoxyyuridin.