African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12364

Full Length Research Paper

Testing public Bt maize events for control of stem borers in the first confined field trials in Kenya

Stephen N. Mugo1*, Murenga Mwimali2, Catherine O. Taracha2, Josephine M. Songa2, Simon T. Gichuki2, Regina Tende2, Haron Karaya1, David J. Bergvinson3, Allesandro Pellegrineschi4, and David A. Hoisington5
1International Maize and Wheat Improvement Center (CIMMYT), P.O. Box 1041 - 00621 Nairobi, Kenya. 2Kenya Agricultural Research Institute (KARI), P.O. Box 57811 – 00200 Nairobi, Kenya. 3Bill & Melinda Gates Foundation (B&MGF), Seattle, Washington, USA. P.O. Box 23350, Seattle, WA 98102. 4Pioneer Hi-Bred International, Inc., P. O. Box 1000, Johnston, IA 50131-0184. 5International Crops Research Institute for Semi-Arid Tropics (ICRISAT), Patancheru 502324, Andhra Pradesh, India.
Email: [email protected]

  •  Accepted: 13 January 2011
  •  Published: 01 June 2011

Abstract

Transgenic  maize (Zea mays L), developed using modified genes from the bacteriumBacillus thuringiensis (Bt), controls stem borers without observable negative effects to humans, livestock or the environment, and is now sown on 134 million hectares globally. Bt maize could contribute to increasing maize production in Kenya. Nine public Bt maize events of cry1Ab and cry1Ba genes were tested in confined field trials site (CFTs) to assess the control of four major Kenyan stem borer species. Leaf damage rating, number of exit holes and tunnel length were scored in the field evaluations. Leaf area consumed and mortality rates among stem borers were scored in the leaf bioassays in a Biosafety Level II laboratory, located at the Kenya Agricultural Research Institute (KARI), National Agricultural Research Laboratories (NARL).  Field evaluations showed that Bt maize controlled Chilo partellus with mean damage scores of 1.2 against 2.7 for the non-Bt CML216 control. Laboratory bioassays showed high control for Eldana saccharina and Sesamia calamistis, with mean larval mortality of 64 and 92%, respectively. However, substantial control was not observed for Busseola fusca. These results showed that Bt maize could control three of the four major stem borers in Kenya with mortality records of 52.7% for B. fusca, 62.3% for E. saccharina and 85.8% for S. calamistis.  Additional Bt genes need to be sought and tested for effective stem borer control in all maize growing ecologies in Kenya.

 

Key words: Maize, Bt, stem borers, confined field trials.

Abbreviation

Bt, Bacillus thuringiensisIPM, integrated pest management; CFTs,confined field trials; KEPHIS, Kenya Plant Health Inspectorate Service.