African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Screening of resistance genes to fusarium root rot and fusarium wilt diseases in F3 family lines of tomato (Lycopersicon esculentum) using RAPD and CAPs markers

Bahattin Tanyolaç* and Cengiz Akkale
Department of Bioengineering, Ege University, 35100 Bornova, Izmir Turkey.
Email: [email protected]

  •  Accepted: 25 January 2010
  •  Published: 10 May 2010

Abstract

 

Fusarium diseases constitute most of the loss in tomato production worldwide, because it spreads on all geographic fields that it is so hard to find a place without fusarium infestation. Thus, the best way to produce tomato is developing resistant cultivars against Fusarium species. In cultivar developing, molecular marker assisted techniques replaced traditional breeding techniques which are high cost and time consuming for breeders. In this study RAPD and CAPS markers were used to screen tomato (Lycopersicon esculentum) lines against resistance genesFrl and I-2, respectivelyResults showed that out of 115 plants, 42 were homozygous resistant, 38 were heterozygous resistant and 35 were homozygous susceptible. Under the light of this information, the forthcoming cultivar development studies will be carried out.

 

Key words: Fusarium oxysporum, marker assisted selection, tomato root rot, CAPs, RAPD.

Abbreviation

RAPD, Randomly amplified polymorphic DNA; CAPS, cleaved amplified polymorphic sequences; FORL, Fusarium oxysporum f. sp. radicis-lycopersici; dNTP, deoxynucleoside 5’-triphosphate; PCR, polymerase chain reaction; bp, nucleotide base pair.