African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12258

Full Length Research Paper

Potential biosurfactant producing endophytic and epiphytic fungi, isolated from macrophytes in the Negro River in Manaus, Amazonas, Brazil

João Marcelo Silva Lima
  • João Marcelo Silva Lima
  • Rede Bionorte de Biodiversidade e Biotecnologia/Universidade Federal do Amazonas (UFAM), Manaus, AM, Brasil.
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José Odair Pereira
  • José Odair Pereira
  • Rede Bionorte de Biodiversidade e Biotecnologia/Universidade Federal do Amazonas (UFAM), Manaus, AM, Brasil.
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Ieda Hortêncio Batista
  • Ieda Hortêncio Batista
  • Universidade do Estado do Amazonas, Manaus, AM, Brasil.
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Pedro de Queiroz Costa Neto
  • Pedro de Queiroz Costa Neto
  • Faculdade de Ciências Agrárias/UFAM, Manaus, AM, Brasil.
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Jucileuza Conceição dos Santos
  • Jucileuza Conceição dos Santos
  • Rede Bionorte de Biodiversidade e Biotecnologia/Universidade Federal do Amazonas (UFAM), Manaus, AM, Brasil.
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Solange Pires de Araújo
  • Solange Pires de Araújo
  • Programa Multi-Institucional de Pós-Graduação em Biotecnologia/UFAM, Manaus, AM, Brasil.
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Mozanil Correia Pantoja
  • Mozanil Correia Pantoja
  • Faculdade de Ciências Agrárias/UFAM, Manaus, AM, Brasil.
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Adolfo José da Mota
  • Adolfo José da Mota
  • Faculdade de Ciências Agrárias/UFAM, Manaus, AM, Brasil.
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João Lúcio de Azevedo
  • João Lúcio de Azevedo
  • Rede Bionorte de Biodiversidade e Biotecnologia/Universidade Federal do Amazonas (UFAM), Manaus, AM, Brasil.
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  •  Received: 27 November 2015
  •  Accepted: 23 May 2016
  •  Published: 15 June 2016

Abstract

Endophytic and epiphytic fungi isolated from Eichhornia crassipes (Mart.) Solms and Cyperus ligularis L., macrophytes collected from oil-contaminated waters, were studied to assess their potential for producing biosurfactants; the most promising ones were identified by means of the rDNA region sequencing. In the selection, in the hydrocarbonate biodegradation activity, 2.6-indophenol (DCPIP) in oil-added Bushnell-Haas (BH) medium was the indicator used. The following tests were performed to ascertain the biosurfactant, bioemulsifier activity: emulsification measurement, drop-collapse, surface tension and production slope. Of the twenty fungi isolated, six promoted DCPIP discoloration. The isolate (S31) Phoma sp. showed emulsification of diesel (1.5 cm or 52%) and reduction of the surface tension of 51.03 mN/m water identified as Phoma sp. The other five fungi were identified as Rhizopus oryzae (S24), Fusarium sp. (S32, S33, S42, S46), presenting potential for biodegradation of hydrocarbons, as well. New studies on Phoma sp. (S31), including its cultivation in different carbon sources will be necessary to improve the production of secondary compounds involved in surface tension bioemulsification and reduction.

Key words: Bioremediation, bioemulsifiers, Eichhornia, Cyperus, oil, diesel.