African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12184

Full Length Research Paper

Establishing sterilization protocol and shoot induction medium for in vitro regeneration of sweetbriar (Rosa rubiginosa L.)

Miloane Meshack Mokhobo
  • Miloane Meshack Mokhobo
  • Department of Molecular Biology and Biotechnology, Pan African University Institute of Basic Sciences, Technology and Innovation, Nairobi, Kenya
  • Google Scholar
Remmy Wekesa Kasili
  • Remmy Wekesa Kasili
  • Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
  • Google Scholar
Amos Emitati Alakonya
  • Amos Emitati Alakonya
  • Institute for Biotechnology Research, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
  • Google Scholar


  •  Received: 05 April 2017
  •  Accepted: 12 May 2017
  •  Published: 31 May 2017

Abstract

Rosa rubiginosa L. is popular in Lesotho where rural communities harvest the rosehips for sale to the local companies which process them into various products. There is, however, a challenge in establishing plantations of the species due to deep seed dormancy and so difficulty in germination. In this study, nodal explants of R. rubiginosa L. were sterilized with 20 g·L-1 Ridomil Gold MZ (RGM) and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP). The explants treated with 20 g·L-1 RGM showed resistant against fungal contamination giving 73% survival explants. Moreover, addition of BAP in shoot induction medium increased shoot formation as compared to 29% response recorded on MS without BAP. Response of 100% shoot formation was obtained on MS medium with 2 mg·L-1 BAP.

 

Key words: 6-Benzylaminopurine, nodal explants, ridomil gold mz.