African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Development of simple kinetic models and parameter estimation for simulation of recombinant human serum albumin production by Pichia pastoris

Panchiga Chongchittapiban
  • Panchiga Chongchittapiban
  • Department of Chemical Engineering, Faculty of Engineering King Mongkut's University of Technology Thonburi (KMUTT), 126 Pracha-utid Road, Bangmod, Toongkru, Bangkok 10140, Thailand.
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Jӧrgen Borg
  • Jӧrgen Borg
  • Pilot Plant Development and Training Institute (PDTI), 49 Soi Tientalay 25, Bangkhuntien-Chaithalay Road, Thakham, Bangkhuntien, Bangkok 10150, Thailand.
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Yaowapha Waiprib
  • Yaowapha Waiprib
  • Department of Fishery Products, Faculty of Fisheries, Kasetsart University (KU), 50 Ngam Wong Wan Road, Ladyaow, Chatuchak, Bangkok 10900, Thailand.
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Jindarat Pimsamarn
  • Jindarat Pimsamarn
  • Department of Chemical Engineering, Faculty of Engineering King Mongkut's University of Technology Thonburi (KMUTT), 126 Pracha-utid Road, Bangmod, Toongkru, Bangkok 10140, Thailand.
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Anan Tongta
  • Anan Tongta
  • Division of Biotechnology, School of Bioresources and Technology King Mongkut’s University of Technology Thonburi (KMUTT), 49 Soi Tientalay 25, Bangkhuntien-Chaithalay Road, Thakham, Bangkhuntien, Bangkok 10150, Thailand.
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  •  Received: 23 November 2015
  •  Accepted: 07 September 2016
  •  Published: 28 September 2016

Abstract

In order to describe and predict the growth and expression of recombinant proteins by using a genetically modified Pichia pastoris, we developed a number of unstructured models based on growth kinetic equation, fed-batch mass balance and the assumptions of constant cell and protein yields. The growth of P. pastoris on both glycerol and methanol could be represented by Monod kinetic equation. A simple simulation methodology and developed models were shown to satisfactorily describe both growth and production of recombinant human serum albumin (rHSA) using a genetically modified P. pastoris MutS strain. The obtained parameters from curve fitting were reasonable and could be acceptable. Moreover, the same parameter sets obtained by the experiments indicated the rigidity and consistency of the developed models and fermentation approach of this study. With correlation coefficients (r2) exceeding 0.99, the models were able to simulate and predict the cell growth behavior and recombinant protein production by P. pastoris without requiring complex models.

 

Key words: Exponential feed, growth modeling, Monod kinetic equation, Pichia pastoris, recombinant human serum albumin.