African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12213

Full Length Research Paper

Synergistic action of Azospirillum brasilense combined with thiamethoxam on the physiological quality of maize seedlings

Andre Gustavo Battistus*
  • Andre Gustavo Battistus*
  • State University of West Parana, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Parana, Brasil.
  • Google Scholar
Tiago Luan Hachmann
  • Tiago Luan Hachmann
  • State University of West Parana, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Parana, Brasil.
  • Google Scholar
Thaisa Muriel Mioranza
  • Thaisa Muriel Mioranza
  • State University of West Parana, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Parana, Brasil.
  • Google Scholar
Mônica Anghinoni Muller
  • Mônica Anghinoni Muller
  • State University of West Paraná, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Paraná, Brasil.
  • Google Scholar
Tiago Madalosso
  • Tiago Madalosso
  • State University of West Paraná, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Paraná, Brasil.
  • Google Scholar
Patricia Aparecida Favorito
  • Patricia Aparecida Favorito
  • State University of West Parana, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Parana, Brasil.
  • Google Scholar
Vandeir Francisco Guimaraes
  • Vandeir Francisco Guimaraes
  • Pontifical Catholic University of Paraná, Avenue of Union, Number 500, Zip Code: 85902-532, Jardim Coopagro.Toledo, Paraná, Brasil.
  • Google Scholar
Jeferson Klein
  • Jeferson Klein
  • Pontifical Catholic University of Paraná, Avenue of Union, Number 500, Zip Code: 85902-532, Jardim Coopagro.Toledo, Paraná, Brasil.
  • Google Scholar
Debora Kestring
  • Debora Kestring
  • State University of West Parana, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Parana, Brasil.
  • Google Scholar
Adriano Mitio Inagaki
  • Adriano Mitio Inagaki
  • State University of West Parana, Pernambuco street, Number 1777, Zip Code: 85960-000, Box: 91, Marechal Cândido Rondon, Parana, Brasil.
  • Google Scholar
Lucas Guilherme Bulegon
  • Lucas Guilherme Bulegon
  • Lucas Guilherme Bulegon1
  • Google Scholar


  •  Received: 20 July 2014
  •  Accepted: 25 November 2014
  •  Published: 03 December 2014

Abstract

Plant growth promoting substances, either from biological or chemical origin, are widely used in modern agriculture. In this context, this study evaluated the physiological quality of maize seed treated with thiamethoxam and inoculated with Azospirillum brasilense. Initially, we tested bacterial growth on medium with increasing concentrations of thiamethoxam. We also evaluated the physiological quality of seeds through morphometric measurements, emergence speed index and chlorophyll content by SPAD through a 2x3 factorial arrangement with seed inoculation with A. brasilense (100 mL per 25 kg of seed) and three doses of thiamethoxam (0, 80 and 120 ml per 60,000 seeds). Bacterial population showed a linear reduction according to increasing doses of thiamethoxam. The dose of 120 ml thiamethoxam (42 mg active ingredient) caused a decrease in several variables, while, the inoculation proved a positive activity in seedlings physiological quality. Thus, the inoculation combined with 80 ml thiamethoxam (28 mg active ingredient) show synergistic action in early development in maize seedlings.

 

Key words: Thiamethoxam, inoculant, plant growth promoting bacteria.