African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12191

Full Length Research Paper

LDH and G-6PDH activities in the ovaries of adult female Wistar rats following the administration of aqueous extracts of neem (Azadirachta indica) leaves

A. J. David1, G. A. Adefolaju1*, B. U. Enaibe1, M. S. Ajao2 and E. A. Caxton-Martins1
  1Anatomy Department, Faculty of Basic Medical Sciences, College of Health Sciences University of Ilorin, Ilorin, Nigeria. 2School of Anatomical Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Email: [email protected],[email protected],[email protected]

  •  Accepted: 03 February 2009
  •  Published: 06 April 2009

Abstract

 

The present study was designed to evaluate the effects of aqueous extracts of neem (Azadirachta Indica) leaves (which have been documented for its antifertility effect on experimental animals) on glucose-6-phosphate dehydrogenase (G-6PDH) and lactate dehydrogenase (LDH) levels in the ovaries of adult female wistar rats. Twenty four adult female wistar rats weighing 200 ± 10 g were divided into three groups A, B and C of eight animals each. Groups A and B were given 3 and 6 mg/kg body weight of extract respectively and the control group was given water orally for 21 days, at the end of which the animals were sacrificed and their ovaries assayed spectrophotometrically for the activities of G-6PDH and LDH. There was significant (p = 0.046) decrease in G-6-PDH and significant (p = 0.047) increase in LDH enzyme activities in the administered groups. The results indicate that extracts of neem which is widely consumed for a variety of ailments alters carbohydrate metabolism in the ovarian tissue.

 

Key words: Neem, ovary, lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PDH).