An experimental effluent analysis was conducted in conjunction with bioremediation process from Mario-jose tannery industry, stream and reference water samples from well (Yandoka), respectively, were collected from the industrial areas. Physiochemical characteristic of these samples were determined viz; chemical oxygen demand (COD), biochemical oxygen demand (BOD), pH, temperature etc. The effluent values were mostly observed higher than the reference samples. Spectrophotometric method using 1,5 dipheyl carbazide as a colour developing reagent has been used to analyzed the chromium (VI) content in tannery effluent. Aspergillius niger, Rhizopus nigrians and Penicillium sp singly and in combination were used for the biosorption studies. Chi- square test analysis for fitness shows that the concentration of the media were (Fcal 0.05) for PDM and (Fcal 0.102) for MRHM varies significantly (α=0.05). Hence, the absorptions ability was highly effective on MRHM than the PDM. From the experimental observation, it has been found that the level of chromium (VI) can be quantitatively reduced at the equilibrium pH range from 5.2-8.0.
Key words: Biosorption, chromium (VI), Aspergillus niger, Rhizopus nigrican, Penicillium sp., agricultural waste, 1,5 dipheyl carbazide.
COD, Chemical oxygen demand; BOD, biochemical oxygen demand; SmF, submerged fermentation; SSF, solid state fermentation.