African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Research on the esterification property of esterase produced by Monascus sp.

MaoBin Chen1, Hong Liu1, Da Zhen and Shangling Fang2*        
1Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Key Laboratory of Industrial Microbiology, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China 2College of Bioengineering, Hubei University of Technology, Wuhan, 430068, China.
Email: [email protected]

  •  Accepted: 26 April 2011
  •  Published: 13 June 2011

Abstract

Esterification reaction by Monascus was produced with a mixed culture of different strains in which Monascus play a major role with higher activity and good specificity. The strain Q-306 was identified in the study as Monascus sp. with high esterification power usage and was screened from Daqu and Zhaopei in China. The esterification characteristic research showed the strain’s specificity to ethyl caproate. On the basis of the single factor experiment, the optimal condition of producing esterification was as follows: temperature was 35°C, alcohol concentration was 20% (v/v), pH value was 3.5 and substrate concentration was 1%.

 

Key words: Esterification Monascus, esterifying power, catalysis characteristic.