African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Cytotoxicity and antibacterial studies of iridoids and phenolic compounds isolated from the latex of Himatanthus sucuuba

Jefferson Rocha A. Silva1*, Claudia M. Rezende2, Angelo C. Pinto2 and Ana Claudia F. Amaral3
1Departamento de Química, Universidade Federal do Amazonas, Av. Rodrigo Otávio, 3000 - Japiim - Manaus / AM, 69077-000, Brazil. 2Instituto de Química, UFRJ, Rio de Janeiro / RJ, 21945-970, R J, Brazil. 3Laboratório de Plantas Medicinais e Derivados, Farmanguinhos/FIOCRUZ – R. Sizenando Nabuco, 100 - Manguinhos / RJ, 21041-250, RJ, Brazil.
Email: [email protected], [email protected]

  •  Accepted: 20 August 2010
  •  Published: 25 October 2010

Abstract

The latex of Himatanthus sucuuba (Spruce) Woodson, used popularly in the Amazon for the treatment of tumors, gastritis, inflammations and infections, was evaluated for cytotoxicity and antibacterial activities. The iridoid lactones, plumericin and isoplumericin were isolated from latex by bioassay fractionation and were found to be associated with DNA damage. Gallic acid exhibited the highest antimicrobial activity among the phenolic compounds isolated from the aqueous fraction. The compounds associated to cytotoxicity and antimicrobial activities could be responsible to the effects of this species used in traditional medicine.

 

Key words: Himatanthus sucuuba, iridoids, phenolics, cytotoxicity, antibacterial.

Abbreviation

TLC, Thin layer chromatography; MIC, minimum inhibitory concentration; IC12, concentration (µg/ml) is required to produce a zone of inhibition of 12 mm; MeOH, methanol; EtOAc, ethyl ethanoate; n-BuOH, n-butanol; GC/MS,gas chromatography coupled with mass spectrophometer; NMR, nuclear magnetic resonance; MPLC, medium pressure liquid chromatography.