African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12227

Full Length Research Paper

Accelerated in vitro propagation of elite oil palm genotypes (Elaeis guineensis Jacq.) by substituting cytokinin with putrescine

Thais Roseli Correa
  • Thais Roseli Correa
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Sérgio Yoshimitsu Motoike
  • Sérgio Yoshimitsu Motoike
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Ana Paula de Souza Andrade
  • Ana Paula de Souza Andrade
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Sara Morra Coser
  • Sara Morra Coser
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Vanessa Queiroz
  • Vanessa Queiroz
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Manuela Maria Cavalcante Granja
  • Manuela Maria Cavalcante Granja
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Débora Durso Neves Caetano
  • Débora Durso Neves Caetano
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Cristian Nicolás Mendoza Pena
  • Cristian Nicolás Mendoza Pena
  • Departamento de Fitotecnia, Universidade Federal de Viçosa, MG, Brazil.
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Edgard Augusto de Toledo Picoli
  • Edgard Augusto de Toledo Picoli
  • Departamento de Biologia Vegetal, Universidade Federal de Viçosa, MG, Brazil.
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  •  Received: 15 September 2016
  •  Accepted: 23 November 2016
  •  Published: 14 December 2016

Abstract

Clonal multiplication of oil palm enables the formation of commercial plantations with higher yield, which is possible through somatic embryogenesis. However, due to different modifications made in the existing protocols to avoid the appearance of the “mantled flower”, the success of this technique has been limited. Thus, this study aimed to apply a cloning protocol, using somatic embryogenesis, to 32 elite oil palm genotypes from a commercial plantation on which cytokinin was substituted with putrescine. All tested genotypes responded positively to callus induction. Moreover, the percentages of responses were differentiated: 65.62% of these genotypes produced embryogenic lines, and 40.62% out of that percentage presented lines with moderate or high multiplication capacity, which is the main factor that enabled the obtaining of clones to form a commercial clonal plantation. The somatic embryogenesis was efficient, making this protocol applicable at a commercial scale, since it allows the obtaining of clones up to 360 days. However, future evaluations on these clones are needed to investigate the appearance of the “mantled flower”.

 

Key words: Cloning, polyamines, tissue culture, commercial plantation.