African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12072

Full Length Research Paper

Antioxidant activity and acute toxicity of Neoglaziovia variegata (Bromeliaceae)

Sarah Raquel Gomes de Lima-Saraiva1, Amanda Leite Guimarães2, Ana Paula de Oliveira2, Henrique César Costa Saraiva2, Raimundo Gonçalves de Oliveira-Júnior2, Vanessa Raquel P. de Barros2, Vanúzia G. Menezes2, Roniere Alencar de Oliveira2, Fabrício Sousa Silva2, Ricardo Santana de Lima2, Maria Helena Tavares de Matos2, Elba Lúcia Cavalcanti Amorim1 and Jackson Roberto Guedes da Silva Almeida2*
1Federal University of Pernambuco, 50.740-521, Recife, Pernambuco, Brazil.2Federal University of San Francisco Valley, 56.304-205, Petrolina, Pernambuco, Brazil.
Email: [email protected]

  •  Accepted: 23 July 2012
  •  Published: 18 September 2012

Abstract

Antioxidant activities of Neoglaziovia variegata were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching and was compared with ascorbic acid, butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The total phenolics content of the extracts was determined by the Folin-Ciocalteu method. Total flavonoid was also determined. The most significant total phenolic content was of 543.50 ± 9.38 mg of gallic acid equivalent/g for ethyl acetate extract (AcOEt), which presented the best antioxidant activity (IC50 5.08 ± 0.20 µg/ml) for DPPH scavenging. The acute toxicity of Nv-EtOH was performed 2.0 g/kg intraperitoneally and 5.0 g/kg orally in mice. No mortality and no toxicity signs were observed, indicating low toxicity of the extract. Blood was removed after 14 days for laboratory analysis of hematological and biochemical parameters. Alterations ofaspartate aminotransferase (AST) and creatinine were observed. The data obtained showed that the doses induced microscopic alterations in the liver and kidney. In conclusion, the Nv-EtOH can be considered of low toxicity.

 

Key words: Antioxidant activity, acute toxicity, Neoglaziovia variegata, Bromeliaceae

Abbreviation

 DPPH, 2,2-Diphenyl-1-picrylhydrazyl; BTH, butylated hydroxytoluene;BHA, butylated hydroxyanisole; AcOEt, ethyl acetate extract.

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