African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Seed storage protein polymorphism in ten elite rice (Oryza sativa L.) genotypes of Sindh

S. Galani1*, F. Naz1, F. Soomro1, I. Jamil1, Zia-ul-hassan2, A. Azhar1 and A. Ashraf3
  1Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Pakistan. 2Sindh Agriculture University, Tandojam, Pakistan. 3Department of Bioinformatics and Biotechnology, G. C. University, Faisalabad, Pakistan.
Email: [email protected]

  •  Accepted: 30 December 2010
  •  Published: 14 February 2011

Abstract

 

Seed protein profiling is the most promising tool in determining the molecular polymorphism and genetic homology. Seed storage proteins help in cultivar identification by avoiding the external environmental influences. Electrophoretically detectable proteins in rice grains possess the potential of characterizing the germplasms by their taxonomic and evolutionary aspects. This study was aimed at exploiting the genetic variations among ten elite rice genotypes of Sindh through electrophoretical separation of grain proteins by sodium dodecyl sulphate polyacryamide gel electrophoresis (SDS-PAGE). In conclusion, the investigation revealed negligible polymorphism, with reference to the total seed protein profiles, among the rice genotypes of Sindh, Pakistan used in this study. Hence, it is highly important to include a significant number of rice genotypes to explore their existing genotypic diversity for future rice breeding programs. The SDS-PAGE in combination with 2-D electrophoresis is further suggested for documenting contrasting variations of isoforms of protein peptides.

 

Key words: Seed storage proteins, polymorphism, Oryza sativa, Sindh, SDS-PAGE

  • Articles on Google by: