African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

In vitro propagation of some promising genotypes of jojoba (Simmondsia chinensis)

Muhammad Azhar Bashir1, Muhammad Akbar Anjum2 and Hamid Rashid3
  1Horticultural Research Station, Bahawalpur, 63100 Pakistan. 2University College of Agriculture, Bahauddin Zakariya University, Multan - 60800 Pakistan. 3Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad Campus, Islamabad, Pakistan.
Email: [email protected]

  •  Accepted: 26 September 2008
  •  Published: 05 November 2008

Abstract

 

Nodal segments (1.5 - 3.0 cm long) of six promising jojoba genotypes were culturedin vitro on solidified MS medium supplemented with BA in combination with different concentrations of NAA, IAA or IBA for shoot formation. The plant growth regulators combination of 5.55 µM BA + 7.1 µM IAA proved the best for shoot initiation and subsequent growth. Some explants of the jojoba genotypes, PKJ-3 and PKJ-6, developed thick rootlets when BA (5.55 µM) was used in combination with IBA (6.1 µM) or NAA (6.7 µM). Many explants developed callus on the medium containing BA (11.1 µM) in combination with NAA (13.4 µM), IAA (14.3 µM) or IBA (12.2 µM). In vitroderived shoots were rooted by culturing on solidified MS medium containing IBA (6.1 µM), IAA (7.1 µM) or NAA (6.7 µM). The plantlets which developed roots in response to IBA had the maximum survival percentage (63.33) during acclimatization in greenhouse. However, survival of the plantlets in field conditions was independent of the genotypes and the time of planting. Among the genotypes, PKJ-3 performed the best in all the parameters studied.

 

Key words: Auxins, cytokinins, genotypes, jojoba, micropropagation, Simmondsia chinensis.

Abbreviation

BA, N6-Benzyladenine; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid; NAAa-napthalene acetic acid; PGRs, plant growth regulators.