African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12209

Full Length Research Paper

Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut

Maria Isabel G. Martins
  • Maria Isabel G. Martins
  • Northeast Biotechnology Network RENORBIO UFRPE, Brazil.
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Antonio Euzebio G. Sant'Ana
  • Antonio Euzebio G. Sant'Ana
  • Chemistry Department, University Federal of Alagoas UFAL, Brazil.
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Felipe Matheus T. Vasconcelos
  • Felipe Matheus T. Vasconcelos
  • Agronomy Department, University Federal Rural of Pernambuco UFRPE, Brazil.
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Wbyratan L. Silva
  • Wbyratan L. Silva
  • Chemistry Department, University Federal of Alagoas UFAL, Brazil.
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Liziane M. Lima
  • Liziane M. Lima
  • Embrapa Algodão, Rua Osvaldo Cruz, 1143, Centenário, CEP:58428-095, Campina Grande, PB, Brazil.
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Reginaldo Carvalho*
  • Reginaldo Carvalho*
  • Federal Rural University of Pernambuco, Botany Graduate Program, 52.171-900, Dois Irmãos, Recife, Pernambuco, Brazil.
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Pericles A. Melo Filho
  • Pericles A. Melo Filho
  • Agronomy Department, University Federal Rural of Pernambuco UFRPE, Brazil.
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Roseane C. Santos
  • Roseane C. Santos
  • Embrapa Algodão, Rua Osvaldo Cruz, 1143, Centenário, CEP:58428-095, Campina Grande, PB, Brazil.
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  •  Received: 02 November 2015
  •  Accepted: 19 July 2016
  •  Published: 27 July 2016

Abstract

Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut.

Key words: Basil, chromatography, eugenol, eugenol synthase, qPCR, spider mite.

Abbreviation

PCR, Polymerase chain reaction; ISSR, inter simple sequence repeat; UBC, University of British Columbia; UPGMA, unweighted pair group method to obtain an arithmetic mean; NCBI, National Center for Biotechnology Information; RT, room temperature; BOD, biochemical oxygen demand; DAE, days after emergence.