African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Analysis of phosphoric ore bacterial and eucaryal microbial diversity by single strand conformation polymorphism (SSCP) and small-subunit (SSU) sequencing

Ilhem Omri*
  • Ilhem Omri*
  • Laboratoire d’Ecologie et de Technologie Microbienne. Department de Génie Chimique et Biologique. Institut Nationale des Sciences Appliquées et de Technologie (INSAT). B. P 676, 1080.University of Carthage, Tunis, Tunisia.
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Imen Manaii
  • Imen Manaii
  • Laboratoire d’Ecologie et de Technologie Microbienne. Department de Génie Chimique et Biologique. Institut Nationale des Sciences Appliquées et de Technologie (INSAT). B. P 676, 1080.University of Carthage, Tunis, Tunisia.
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Zied Tlili
  • Zied Tlili
  • Higher Institute of Business Administration of Gafsa, Tunisia.
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Jean-Jacques Godon
  • Jean-Jacques Godon
  • INRA UR50, Laboratoire de Biotechnologie de l’Environnement, Avenue des Étangs, F-11100, Narbonne, France.
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Moktar Hamdi
  • Moktar Hamdi
  • Laboratoire d’Ecologie et de Technologie Microbienne. Department de Génie Chimique et Biologique. Institut Nationale des Sciences Appliquées et de Technologie (INSAT). B. P 676, 1080.University of Carthage, Tunis, Tunisia.
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  •  Received: 30 July 2013
  •  Accepted: 04 July 2014
  •  Published: 23 July 2014

Abstract

The aim of this study was to investigate the phosphoric ore bacterial and eucaryal microbial diversity by using the single strand conformation polymorphism (SSCP) technique and small-subunit (SSU) sequencing. PCR-SSCP patterns showed a remarkably simple microbial community, mainly for bacterial community, but also for the eukaryotic community. According to the highly bacterial variable V3 region of 16S rRNA sequences, five bacterial species were identified: an unidentified species belonged to proteo gamma phylum, an unidentified species belonging to proteo delta phylum, one belonged to Moraxellaceae and two closed to Pseudomonadaceae as predominant bacteria in the mining residue. The study showed for the first time that phosphoric ore harbors major bacterial groups related to organisms having a wide range of environmentally significant functional attributes. These findings provided new opportunities into phosphoric ore microbiology that could be useful in biological system removing waste gases generated from the phosphoric industry.

 

Key words: Microbial community, bacteria, archaea, eucarya, mining residue.

Abbreviation

Abbreviations: H2S, Hydrogen sulfide; PCR, polymerase chain reaction; SSCP, single strand conformation polymorphism; SSU, small subunit; VOC, volatile organic compound.