African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12254

Full Length Research Paper

Detection of extracellular enzymatic activity in microorganisms isolated from waste vegetable oil contaminated soil using plate methodologies

Eugenia G. Ortiz Lechuga
  • Eugenia G. Ortiz Lechuga
  • Facultad de Ciencias Biológicas, Instituto de Biotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba y Manuel Barragán s/n. Ciudad Universitaria. C. P. 66455. San Nicolás de los Garza, Nuevo León, México.
  • Google Scholar
Isela Quintero Zapata
  • Isela Quintero Zapata
  • Facultad de Ciencias Biológicas, Instituto de Biotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba y Manuel Barragán s/n. Ciudad Universitaria. C. P. 66455. San Nicolás de los Garza, Nuevo León, México.
  • Google Scholar
Katiushka Arévalo Niño⃰
  • Katiushka Arévalo Niño⃰
  • Facultad de Ciencias Biológicas, Instituto de Biotecnología, Universidad Autónoma de Nuevo León, Av. Pedro de Alba y Manuel Barragán s/n. Ciudad Universitaria. C. P. 66455. San Nicolás de los Garza, Nuevo León, México.
  • Google Scholar


  •  Received: 18 September 2015
  •  Accepted: 01 February 2016
  •  Published: 16 March 2016

Abstract

In the present study, from a total of 100 strains isolated from waste vegetable oil contaminated soil, 38 bacterial and 14 fungi strains that presented positive lipolytic activity were obtained by detection through Rhodamine B Agar 0.02% w/v as a screening method. Additionally, two other enzymatic activities were determined. Positive proteolytic activity was evaluated in Casein Hydrolysis Agar and chitinolytic activity was identified by change in coloration in Bromocresol Purple Agar. Using these methodologies, we were able to report 18 microorganisms with two enzymatic activities and 6 microorganisms with all three enzymatic activities, thereby establishing these techniques as suitable and fast approaches for detection and semi-quantification of extracellular enzymatic activity.

Key words: Enzyme, lipases, proteases, chitinases, rhodamine B, soil.